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| Status |
Public on Mar 01, 2022 |
| Title |
RNA-seq analysis of the chromosome-associated RNAs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
The goals of this study are to discover the RNAs associated with the condensed chromosomes during mitosis. RNA from the soluble and pellet fraction of the lysed mitotic HCT116 cells were extracted, RNA profiles of soluble and pellet RNA were generated by deep sequencing, using MGISEQ2000. The sequence reads that passed quality filters were analyzed by STAR (v2.7.8a). Using an optimized data analysis workflow, we found that the pre-rRNAs were significantly enriched in the pellet fraction of the mitotic HCT116 cells.
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| Overall design |
Analyze the RNA from the soluble and pellet fraction of lysed mitotic HCT116 cells
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| Contributor(s) |
Yu X |
| Citation(s) |
35637200, 37582658 |
| BioProject |
PRJNA757928 |
| Submission date |
Aug 29, 2021 |
| Last update date |
Oct 25, 2023 |
| Contact name |
Kai Ma |
| E-mail(s) |
makkay@live.cn
|
| Organization name |
Westlake University
|
| Street address |
No.18 Shilongshan Road，Cloud Town, Xihu District
|
| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
310024 |
| Country |
China |
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| Platforms (1) |
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| Samples (2) |
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