|
| Status |
Public on Sep 01, 2022 |
| Title |
Global miRNA Expression of Bone Marrow Mesenchymal Stem/Stromal Cells in Fanconi Anemia Patients Before Transplantation and Unpaired Bone Marrow Donors |
| Platform organism |
synthetic construct |
| Sample organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
Fanconi anemia (FA) is a rare genetic disorder characterized by genomic instability, developmental defects and bone marrow failure. Homeostasis of hematopoietic stem cells (HSCs) in the bone marrow partly relies on their direct or indirect interactions with the mesenchymal stem/stromal cells (MSCs). miRNAs can play a critical role during these interactions. There is no study available so far addressing the miRNA profile of bone marrow (BM-) MSCs in the FA disease state.
Non-coding RNA expression profiling was performed in BM-MSCs obtained from Donors, as well as FA patients before bone marrow transplantation (preBMT) using GeneChip miRNA 2.0 array. Quality Control (QC) was performed via Normalized Unscaled Standard Errors (NUSE) and Relative Log Expression (RLE) before further analysis.
|
| |
|
| Overall design |
Non-coding RNA expression profiling was performed using total RNAs including small RNAs isolated from passage three BM-MSCs, which are obtained from Donors (n=5 after QC; designated as HUSCS-D) preBMT FA patients (n=4 after QC; designated as HUSCS-FA).
|
| |
|
| Contributor(s) |
Keles M, Keskus AG, Cagnan I, Konu O, Gunel-Ozcan A |
| Citation(s) |
34792755 |
| Submission date |
Sep 01, 2021 |
| Last update date |
Dec 01, 2022 |
| Contact name |
Ayşen Özcan |
| E-mail(s) |
aysen.ozcan@hacettepe.edu.tr, agozcan@yahoo.com
|
| Organization name |
Hacettepe University
|
| Department |
Department of Stem Cell Sciences
|
| Street address |
Sihhiye
|
| City |
Ankara |
| ZIP/Postal code |
06100 |
| Country |
Turkey |
| |
|
| Platforms (1) |
| GPL14613 |
[miRNA-2] Affymetrix Multispecies miRNA-2 Array |
|
| Samples (9)
|
|
| This SubSeries is part of SuperSeries: |
| GSE183227 |
Global miRNA Expression of Bone Marrow Mesenchymal Stem/Stromal Cells in Fanconi Anemia Patients |
|
| Relations |
| BioProject |
PRJNA759641 |
Less...
More...