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| Status |
Public on Sep 08, 2021 |
| Title |
Gene expression in human keratinocytes cultivated with serum-free medium and serum containing medium |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The objective of this study was thus to develop ab initio that chemically defined serum-free medium. We used a statistical design of experiment (DOE) approach to screen a large number of candidate factors, for both their potential individual and synergetic effects, as to identify a formulation which was then tested extensively.
In this study, we present the properties of keratinocyte cultivated with serum-Free medium (Surge SFM) and complemented DME-Ham medium (with serum).
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| Overall design |
Four human keratinocyte populations at passage 2 cultivated with with serum-free medium or serum containing medium until cells attained 80-95% confluence.
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| Contributor(s) |
Cortez Ghio S, Barbier M, Doucet E, Debbah I, Le-Bel G, Cartier A, Jolibois E, Morissette A, Larouche D, Fradette J, Guérin S, Garnier A, Germain L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 07, 2021 |
| Last update date |
Sep 10, 2021 |
| Contact name |
Gaëtan Le-Bel |
| E-mail(s) |
gaetan.lebel17@gmail.com
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| Organization name |
CUO-Recherche
|
| Lab |
Sylvain Guérin
|
| Street address |
Local H2-00, Hôpital du Saint-Sacrement,1050 Chemin Sainte-Foy
|
| City |
Québec |
| State/province |
Quebec |
| ZIP/Postal code |
G1S 4L8 |
| Country |
Canada |
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| Platforms (1) |
| GPL13607 |
Agilent-028004 SurePrint G3 Human GE 8x60K Microarray (Feature Number version) |
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| Samples (8)
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| GSM5563199 |
Cell_Bank_cultured_human_keratinocytes_serum-medium_001_rep1 |
| GSM5563200 |
Cell_Bank_cultured_human_keratinocytes_serum-free-medium_001_rep1 |
| GSM5563201 |
Cell_Bank_cultured_human_keratinocytes_serum-medium_002_rep1 |
| GSM5563202 |
Cell_Bank_cultured_human_keratinocytes_serum-free-medium_002_rep1 |
| GSM5563203 |
Cell_Bank_cultured_human_keratinocytes_serum-medium_003_rep1 |
| GSM5563204 |
Cell_Bank_cultured_human_keratinocytes_serum-free-medium_003_rep1 |
| GSM5563205 |
Cell_Bank_cultured_human_keratinocytes_serum-medium_004_rep1 |
| GSM5563206 |
Cell_Bank_cultured_human_keratinocytes_serum-free-medium_004_rep1 |
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| Relations |
| BioProject |
PRJNA761384 |
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