|
| Status |
Public on Sep 22, 2021 |
| Title |
RNA-seq profiles of germinal center B cells from spleens of wild-type and Il21r-deficient mice after immunization with NP-KLH |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Humoral immune responses require germinal centres (GC) for antibody affinity maturation. Within GC, B-cell proliferation and mutation are segregated from affinity-based positive selection in the dark zone (DZ) and light zone (LZ) substructures, respectively. While IL21 is known to be important in affinity maturation and GC maintenance, here we show it is required for both establishing normal zone representation and preventing the accumulation of cells in G1 cell cycle stage in the GC LZ.
|
| |
|
| Overall design |
Wildtype and IL21 receptor deficient (Il21r-/-) mice were immunized with NP-KLH in alum. Germinal center B cells were extracted from spleens 7 days, 10 days and 14 days after immunization. RNA was extracted and profiled using RNA-seq. An Illumina HiSeq 2000 was used to generate 100bp single-end sequence reads. Each sample was generated from an independent mouse.
|
| |
|
| Contributor(s) |
Smyth GK, Li-Wai-Suen CS, Tarlinton DM, Zotos D |
| Citation(s) |
34887406 |
| Submission date |
Sep 20, 2021 |
| Last update date |
Dec 22, 2021 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL15103 |
Illumina HiSeq 1000 (Mus musculus) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA764732 |
| SRA |
SRP337947 |
Less...
More...