|
| Status |
Public on Oct 13, 2010 |
| Title |
Downregulation of Myc is essential for terminal erythroid maturation |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Terminal differentiation of mammalian erythroid progenitors involves 4-5 cell divisions and induction of many erythroid important genes, followed by chromatin and nuclear condensation and enucleation. The protein levels of c-myc (Myc) are reduced dramatically during late stage erythroid maturation, coinciding with cell cycle arrest in G1-phase and enucleation, suggesting possible roles for c-myc in either or both of these processes. Here we demonstrate that ectopic Myc expression affects terminal erythroid maturation in a dose-dependent manner. Expression of Myc at physiological levels did not affect erythroid differentiation or cell cycle shutdown, but specifically blocked erythroid nuclear condensation and enucleation. Myc prevented deacetylation of several lysine residues in histones H3 and H4 that are normally deacetylated during erythroid maturation. When over-expressed at levels higher than the physiological range, Myc blocked erythroid differentiation and the cells continued to proliferate in cytokine-free, serum-containing culture medium with an early erythroblast morphology. These studies reveal an important dose-dependent function of Myc in regulating terminal maturation in mammalian erythroid cells. Our findings further support the emerging notion that Myc regulates chromatin structure by regulating global histone acetylation states.
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| |
|
| Overall design |
Five groups with three biological replicates in each.
|
| |
|
| Contributor(s) |
Jayapal SR, Lee KL, Ji P, Kaldis P, Lodish HF, Lim B |
| Citation(s) |
20940306 |
| Submission date |
Oct 14, 2009 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Senthil Raja Jayapal |
| E-mail(s) |
senthilraja@gis.a-star.edu.sg
|
| Phone |
+65 64788187
|
| Fax |
+65 6478-9005
|
| Organization name |
Genome Institute of singapore
|
| Department |
Stem cell and developmental biology
|
| Lab |
Stem cell group 4
|
| Street address |
#08-01, Genome, 60 Biopolis Street
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (15)
|
| GSM541245 |
Wild type erythroblasts-uninfected 0hours 1 |
| GSM541246 |
Wild type erythroblasts-uninfected 0hours 2 |
| GSM541247 |
Wild type erythroblasts-uninfected 0hours 3 |
| GSM541248 |
Wild type erythroblasts-uninfected 24hours 1 |
| GSM541249 |
Wild type erythroblasts-uninfected 24hours 2 |
| GSM541250 |
Wild type erythroblasts-uninfected 24hours 3 |
| GSM541251 |
Wild type erythroblasts-uninfected 48hours 1 |
| GSM541252 |
Wild type erythroblasts-uninfected 48hours 2 |
| GSM541253 |
Wild type erythroblasts-uninfected 48hours 3 |
| GSM541254 |
Wild type erythroblasts expressing ectopic Myc at Physiological levels 48hours 1 |
| GSM541255 |
Wild type erythroblasts expressing ectopic Myc at Physiological levels 48hours 2 |
| GSM541256 |
Wild type erythroblasts expressing ectopic Myc at Physiological levels 48hours 3 |
| GSM541257 |
Wild type erythroblasts expressing ectopic Myc at supraphysiological levels 48hours 1 |
| GSM541258 |
Wild type erythroblasts expressing ectopic Myc at supraphysiological levels 48hours 2 |
| GSM541259 |
Wild type erythroblasts expressing ectopic Myc at supraphysiological levels 48hours 3 |
|
| Relations |
| BioProject |
PRJNA121375 |
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