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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 13, 2021 |
| Title |
Effects of murine Oncostatin M on OP9 stromal cells and NIH/3T3 fibroblasts |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The IL-6 cytokine Oncostatin M (OSM) is involved in cell development, growth, hematopoiesis, inflammation and cancer. Intriguingly, OSM has proliferative and antiproliferative effects de-pending on the target cell. The molecular mechanisms underlying these opposing effects are not fully understood and its role in tumorigenesis remains controversial. In the present study, we investigated the effect of murine OSM (mOSM) on proliferation in two cell types of distinct origin. We found that mOSM impairs the proliferation of murine bone marrow (BM) stromal cell lines and primary BM stromal cells, whereas murine fibroblasts responded to mOSM with increased proliferation. When we set out to reveal the mechanisms underlying these opposing effects of OSM on proliferation, we detected increased expression of the OSM receptors OSMR and LIFR in stromal cells. Interestingly, Osmr knockdown and Lifr overexpression attenuated the OSM-mediated effect on proliferation in both cell lines indicating that mOSM affected the pro-liferation of both cell lines signaling mainly through the OSMR. Furthermore, mOSM induced activation of the JAK-STAT, PI3K-AKT and MAPK-ERK pathways in OP9 and NIH/3T3 cells with differences in total protein levels between the two cell lines. Our findings offer new insights into the regulation of proliferation by mOSM.
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| Overall design |
We included 12 samples in total. 3x untreated OP9 cells, 3x OP9 cells + 10 ng/mL of OSM, 3x untreated NIH/3T3 cells, 3x NIH/3T3 cells + 10 ng/mL of OSM.
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| Contributor(s) |
Jakob L, Pfeifer D, Duyster J |
| Citation(s) |
34769079 |
| Submission date |
Oct 11, 2021 |
| Last update date |
Jan 12, 2022 |
| Contact name |
Justus Duyster |
| Organization name |
Freiburg University Medical Center
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| Department |
Department of Hematology and Oncology
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| Lab |
Duyster
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| Street address |
Hugstetter Str. 55
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| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA770266 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE185646_RAW.tar |
16.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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