 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 21, 2010 |
| Title |
Estrogen Coordinates Translation and Transcription Revealing a Role for NRSF in Human Breast Cancer Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Analysis of estrogen receptor (ER)-positive MCF7 cell total RNA expression and polysome-assiciated RNA expression following treatment with estradiol (E2) and vehicle (etoh).
We used expression microarrays to measure polysome association and total RNA abundance in E2 treated MCF7. These data, along with previously published data, show that genes that are upregulated by estrogen treatment are biased towards association with polysomes.
|
| |
|
| Overall design |
MCF7 cells were grown in hormone depleted media for three days before a 1 hour treatment with E2 or 0.1 % ethanol (vehicle). Total RNA was collected using standard methods and polysome-association RNA from the same cells were collected using sucrose gradient fractionation. Both RNA populations were purified, labeled, and hybridized to Affymetrix Human Genest arrays.
|
| |
|
| Contributor(s) |
Brodsky AS, Hillenmeyer S |
| Citation(s) |
20392875 |
| Submission date |
Oct 15, 2009 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Alexander Brodsky |
| E-mail(s) |
alex_brodsky@brown.edu
|
| Phone |
401-444-1649
|
| Organization name |
Rhode Island Hospital/Brown University
|
| Department |
MCB
|
| Lab |
Brodsky lab
|
| Street address |
593 Eddy St.
|
| City |
PROVIDENCE |
| State/province |
RI |
| ZIP/Postal code |
02903 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (18)
|
| GSM462332 |
estrogen treated total RNA, biological rep1 |
| GSM462333 |
estrogen treated total RNA, biological rep2 |
| GSM462334 |
estrogen treated total RNA, biological rep3 |
| GSM462335 |
estrogen treated polysome-associated RNA, biological rep1 |
| GSM462336 |
estrogen treated polysome-associated RNA, biological rep2 |
| GSM462337 |
estrogen treated polysome-associated RNA, biological rep3 |
| GSM462338 |
ethanol (vehicle) treated total RNA, biological rep1 |
| GSM462339 |
ethanol (vehicle) treated total RNA, biological rep2 |
| GSM462340 |
ethanol (vehicle) treated total RNA, biological rep3 |
| GSM462341 |
ethanol (vehicle) treated polysome-associated RNA, biological rep1 |
| GSM462342 |
ethanol (vehicle) treated polysome-associated RNA, biological rep2 |
| GSM462343 |
ethanol (vehicle) treated polysome-associated RNA, biological rep3 |
| GSM533639 |
estrogen treated monosome-associated RNA, biological rep1 |
| GSM533640 |
estrogen treated monosome-associated RNA, biological rep2 |
| GSM533641 |
estrogen treated monosome-associated RNA, biological rep3 |
| GSM533642 |
ethanol (vehicle) treated monosome-associated RNA, biological rep1 |
| GSM533643 |
ethanol (vehicle) treated monosome-associated RNA, biological rep2 |
| GSM533644 |
ethanol (vehicle) treated monosome-associated RNA, biological rep3 |
|
| Relations |
| BioProject |
PRJNA121331 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18592_RAW.tar |
74.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...