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Status |
Public on Nov 01, 2022 |
Title |
The role of miR-199a-3p in epithelial to mesenchymal transition of keratinocytes |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We found that miR-199a-3p expression is decreased in cSCC biopsies and cell lines compared to normal skin biopsies or normal keratinocytes cell. To understand the role lost expression of miR-199a-3p might have in tumorigenesis of keratinocytes, we aimed to fine possible biochemical pathways and possible targets of miR-199a-3p in cSCC. In order do so, we overexpressed miR-199a-3p in cSCC and used microarrays to find global gene expression affected by miR-199a-3p.
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Overall design |
Two cSCC cell lines Met-I and SCL-II were generated, each either over-expressing miR-199a-3p or control plasmid. From these cells, RNA extraction and hybridization on Affymetrix microarrays.
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Contributor(s) |
Mizrahi A, Masalha M, Meningher T, Barzilai A, Gur-Wahnon D, Ben-Dov IZ, Kapenhas J, Jacob-Hirsch J, Leibowitz-Amit R, Sidi Y, Avni D |
Citation(s) |
36499729 |
Submission date |
Oct 18, 2021 |
Last update date |
Jan 05, 2023 |
Contact name |
jasmine Jacob |
E-mail(s) |
j-jacob@sheba.health.gov.il
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Phone |
0523790500
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Organization name |
Sheba Medical Center at Tel HaShomer
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Street address |
haChevel 33
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City |
Shoham |
State/province |
-Select- |
ZIP/Postal code |
60850 |
Country |
Israel |
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Platforms (1) |
GPL15207 |
[PrimeView] Affymetrix Human Gene Expression Array |
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Samples (4)
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Relations |
BioProject |
PRJNA772117 |