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| Status |
Public on Nov 01, 2021 |
| Title |
Prenatal Adversity Alters the Epigenetic Profile of the Prefrontal Cortex: Sexually Dimorphic Effects of Prenatal Alcohol Exposure and Food-related Stress |
| Organism |
Rattus norvegicus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
Prenatal adversity or stress can have long-term consequences on developmental trajec-tories and health outcomes. Although the biological mechanisms underlying these effects are poorly understood, epigenetic modifications, such as DNA methylation, have the potential to link early-life environments to alterations in physiological systems, with long-term functional impli-cations. We investigated the consequences of two prenatal insults, prenatal alcohol exposure (PAE) and food-related stress, on DNA methylation profiles of the rat brain during early devel-opment. As these insults can have sex-specific effects on biological outcomes, we analyzed epige-nome-wide DNA methylation patterns in prefrontal cortex, a key brain region involved in cogni-tion, executive function, and behavior, of both males and females. We found sex-dependent and sex-concordant influences of these insults on epigenetic patterns. These alterations occurred in genes and pathways related to brain development and immune function, suggesting that PAE and food-related stress may reprogram neurobiological/physiological systems partly through central epigenetic changes, and may do so in a sex-dependent manner. Such epigenetic changes may re-flect the sex-specific effects of prenatal insults on long-term functional and health outcomes and may have important implications for understanding possible mechanisms underlying fetal alco-hol spectrum disorder and other neurodevelopmental disorders.
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| Overall design |
DNA methylation data from meDIP-seq of 30 rat samples, spanning three prenatal treatment groups (control, pair-fed, prenatal alcohol exposed) and both sexes. Prefrontal cortex samples were collected on postnatal day 22 (PN22). Each group/sex had n= 5. One sequencing run was performed for each sample (30 aligned bam files) and DNA methylation peaks were called using MACS2 following DNA processing (xls files).
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| Contributor(s) |
Lussier AA, Bodnar TS, Moksa M, Hirst M, Kobor MS, Weinberg J |
| Citation(s) |
34828381 |
| Submission date |
Oct 29, 2021 |
| Last update date |
Dec 09, 2021 |
| Contact name |
Alexandre A Lussier |
| Organization name |
University of British Columbia
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| Department |
Department of Medical Genetics
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| Lab |
Kobor lab
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| Street address |
950 W 28th
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| City |
Vancouver |
| State/province |
British Columbia |
| ZIP/Postal code |
V5Z 4H4 |
| Country |
Canada |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (30)
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| Relations |
| BioProject |
PRJNA776276 |
| SRA |
SRP343757 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE186840_RAW.tar |
121.6 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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