|
| Status |
Public on Nov 29, 2009 |
| Title |
Chromatin Profiling by Directly Sequencing Small Quantities of Immunoprecipitated DNA |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin structure and transcription factor localization can be assayed genome-wide by sequencing genomic DNA fractionated by protein occupancy or other properties. However, current technologies involve multiple steps that introduce bias and inefficiency. Here we apply a single-molecule approach to directly sequence chromatin immunoprecipitated DNA with minimal sample manipulation. This method is accurate, compatible with just 50 picograms of DNA and should thus facilitate charting chromatin maps from limited cell populations.
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| |
|
| Overall design |
Application of a single-molecule approach to directly sequence chromatin immunoprecipitated DNA of the CTCF DNA binding protein.
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| Contributor(s) |
Goren A, Bernstein BE |
| Citation(s) |
19946276 |
| Submission date |
Oct 22, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Alon Goren |
| E-mail(s) |
alon_g@mit.edu
|
| Organization name |
Broad Institute
|
| Street address |
7 Cambridge Center
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142-1401 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| SRA |
SRP001555 |
| BioProject |
PRJNA121485 |
