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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2012 |
| Title |
Expression data from in vitro-induced non-hypoxic tumour associated macrophages (TAMs) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Active immunotherapy is a promising strategy for anti-angiogenic cancer therapy. Recently, we have reported that a vaccine using human umbilical vein endothelial cells (HUVECs) induced specific anti-endothelial immune responses in the most of immunized patients, and resulted in tumor regression in some patients with recurrent malignant brain tumors, whereas not in colorectal cancer patients. In this study, we hypothesized that non-hypoxic perivascular tumor associated macrophages (TAMs) in colorectal cancer, but not in glioblastoma, might negatively alter the therapeutic efficacy of anti-angiogenic active immunotherapy. To test this hypothesis, we examined global gene expression profiles of non-hypoxic macrophages stimulated in vitro by soluble factors released from tumor cells of human glioblastoma U-87MG (‘brain TAMs’) or colorectal adenocarcinoma HT-29 (‘colon TAMs’).
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| Overall design |
Murine non-hypoxic TAMs were induced in vitro by incubation with soluble factors released from human cancer cell lines U-87MG ('brain TAMs') or HT-29 ('colon TAMs'), for RNA extraction and subsequent hybridization on Affymetrix microarrays. To evaluate homogeneous macrophage populations at different tumour developmental stages, RNA aliquots of control macrophages and TAMs obtained at five different time-points, i.e. 8h, 16h, 24h, 32h and 40h, were pooled and used for screening of differentially expressed genes. The experiments for TAMs as well as for control unstimulated macrophages were performed in triplicates.
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| Contributor(s) |
Okaji Y, Tsuno NH, Tanaka M, Sunami E, Kitayama J, Tokunaga K, Nagawa H, Takahashi K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Oct 29, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Yurai Okaji |
| Organization name |
The University of Tokyo
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| Street address |
Bunkyo-ku, Hongo 7-3-1
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| City |
Tokyo |
| ZIP/Postal code |
113-0033 |
| Country |
Japan |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM466436 |
Control macrophage_unconditoned_rep1 |
| GSM466437 |
Control macrophage_unconditoned_rep2 |
| GSM466438 |
Control macrophage_unconditoned_rep3 |
| GSM466439 |
Brain TAMs_human glioblastoma-conditioned_rep1 |
| GSM466440 |
Brain TAMs_human glioblastoma-conditioned_rep2 |
| GSM466441 |
Brain TAMs_human glioblastoma-conditioned_rep3 |
| GSM466442 |
Colon TAMs_human colorectal adenocarcinoma-conditioned_rep1 |
| GSM466443 |
Colon TAMs_human colorectal adenocarcinoma-conditioned_rep2 |
| GSM466444 |
Colon TAMs_human colorectal adenocarcinoma-conditioned_rep3 |
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| Relations |
| BioProject |
PRJNA121217 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18804_RAW.tar |
36.7 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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