|
| Status |
Public on Nov 25, 2009 |
| Title |
Discovering Hematopoietic Mechanisms Through Genome-Wide Analysis of GATA Factor Chromatin Occupancy |
| Project |
ENCODE
|
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
Expression profiling by array
|
| Summary |
GATA factors interact with simple DNA motifs (WGATAR) to regulate critical processes, including hematopoiesis, but very few WGATAR motifs are occupied in genomes. Given the rudimentary knowledge of mechanisms underlying this restriction, and how GATA factors establish genetic networks, we used ChIP-seq to define GATA-1 and GATA-2 occupancy genome-wide in erythroid cells. Coupled with genetic complementation analysis and transcriptional profiling, these studies revealed a rich collection of targets containing a characteristic binding motif of greater complexity than WGATAR. GATA factors occupied loci encoding multiple components of the Scl/TAL1 complex, a master regulator of hematopoiesis and leukemogenic target. Mechanistic analyses provided evidence for cross-regulatory and autoregulatory interactions among components of this complex, including GATA-2 induction of the hematopoietic corepressor ETO-2 and an ETO-2 negative autoregulatory loop. These results establish fundamental principles underlying GATA factor mechanisms in chromatin and illustrate a complex network of considerable importance for the control of hematopoiesis.
For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODEDataReleasePolicyFinal2008.pdf
This SuperSeries is composed of the SubSeries listed below.
|
| |
|
| Overall design |
Refer to individual Series.
|
| Web link |
http://www.ncbi.nlm.nih.gov/geo/info/ENCODE.html
|
| |
|
| Contributor(s) |
Fujiwara T, O'Geen H, Keles S, Blahnik K, Linnemann AK, Kang Y, Choi K, Farnham PJ, Bresnick EH |
| Citation(s) |
19941826 |
| BioProject |
PRJNA63447 |
| Submission date |
Oct 30, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Emery H. Bresnick |
| E-mail(s) |
ehbresni@facstaff.wisc.edu
|
| Phone |
608-265-6446
|
| Fax |
608-262-1257
|
| URL |
http://www.wisc.edu/molpharm/faculty/bresnick.html
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Pharmacology
|
| Lab |
Bresnick Lab
|
| Street address |
1300 University Avenue 383 MSC
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL6103 |
Illumina mouseRef-8 v1.1 expression beadchip |
| GPL9115 |
Illumina Genome Analyzer II (Homo sapiens) |
|
| Samples (11)
|
| GSM467714 |
Estradiol minus_replicate 1_hybridization 1 |
| GSM467715 |
Estradiol plus_replicate 1_hybridization 1 |
| GSM467716 |
Estradiol minus_replicate 2_hybridization 1 |
| GSM467717 |
Estradiol plus_replicate 2_hybridization 1 |
| GSM467718 |
Estradiol minus_replicate 1_hybridization 2 |
| GSM467719 |
Estradiol plus_replicate 1_hybridization 2 |
| GSM467720 |
Estradiol minus_replicate 2_hybridization 2 |
| GSM467721 |
Estradiol plus_replicate 2_hybridization 2 |
|
| This SuperSeries is composed of the following SubSeries: |
| GSE18868 |
Genome-wide maps of GATA factor occupancy in K562 cells |
| GSE18870 |
Analysis of global gene expression in uninduced and β-estradiol treated G1E-ER-GATA cells |
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