|
| Status |
Public on Nov 10, 2009 |
| Title |
mRNA abundance in HEK293T cells mock or miR-124 transfected |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
HEK293T cells were either mock or miR-124 transfected. Cells were lysed 12 hours after transfection with Trizol reagent. RNA was isolated, amplified, labeled (cy5), and comparatively hybridized with a universal reference (cy3) on HEEBO arrays in biological triplicate. In addition, each biological replicate was measured twice (#2's). Data from the technical replicates was averaged before analysis. SS=steady state
Compound Based Treatment: miR-124
|
| |
|
| Overall design |
Set of arrays that are part of repeated experiments
|
| |
|
| Contributor(s) |
Hogan D |
| Citation(s) |
19901979 |
| Submission date |
Oct 30, 2009 |
| Last update date |
Mar 21, 2012 |
| Contact name |
Patrick O Brown |
| E-mail(s) |
pbrown@cmgm.stanford.edu
|
| Organization name |
Stanford university school of medicine
|
| Department |
Department of biochemistry
|
| Street address |
Beckman Center b439
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL9494 |
SMD Print_1539 Homo sapiens |
| GPL9497 |
SMD Print_1529 Homo sapiens |
|
| Samples (12)
|
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| This SubSeries is part of SuperSeries: |
| GSE18889 |
Concordant Regulation of Translation and mRNA Abundance for Hundreds of Targets of a Human microRNA |
|
| Relations |
| BioProject |
PRJNA123845 |
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