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| Status |
Public on Jan 01, 2010 |
| Title |
Let-7c and miR-294 target identification in mouse ES cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
let-7c and miR-294 were transfected into Dgcr8 -/- miRNA deficient ES cells and RNA was harvested after 12 hours
the goal of this study was to identify direct and indirect targets of the let-7c and miR-294 miRNAs, we chose to harvest RNA early at 12 hours to mimize secondary effects due to ES cell differentiation, prior to performing the array experiment we found that at this time point candidate miRNA targets were maximally downregulated by qPCR
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| Overall design |
9 samples were analyzed, 3 biological replicates each of mock transfected, let-7c transfected, and miR-294 transfected
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| Contributor(s) |
Melton CA, Blelloch RH |
| Citation(s) |
20054295 |
| Submission date |
Oct 31, 2009 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Robert Blelloch |
| E-mail(s) |
blellochr@stemcell.ucsf.edu
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| Phone |
415-476-2838
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| Organization name |
University of California, San Francisco
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| Street address |
513 Parnassus Avenue, Campus Box #0525
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| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| GSM466925 |
let-7c transfected, biological replicate 1 |
| GSM466926 |
let-7c transfected, biological replicate 2 |
| GSM466927 |
let-7c transfected, biological replicate 3 |
| GSM466928 |
miR-294 transfected, biological replicate 1 |
| GSM466929 |
miR-294 transfected, biological replicate 2 |
| GSM466930 |
miR-294 transfected, biological replicate 3 |
| GSM466931 |
mock transfected, biological replicate 1 |
| GSM466932 |
mock transfected, biological replicate 2 |
| GSM466933 |
mock transfected, biological replicate 3 |
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| Relations |
| BioProject |
PRJNA121175 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE18840_RAW.tar |
41.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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