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| Status |
Public on Jan 01, 2010 |
| Title |
Novel IL-21 signaling pathway upregulates c-Myc and induces apoptosis of diffuse large B-cell lymphomas |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Diffuse large B cell lymphoma is a heterogenous malignancy that is diagnosed in over 60,000 patients each year. Interleukin-21 has been shown to induce apoptosis in certain B cells originating from mice. Diffuse large B cell lyphomas express the Interleukin-21 Receptor yet the effects of Interleukin-21 on this malignancy are uknown. Identified genes responsive to IL-21 treatment in diffuse large B cell lymphoma.
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| Overall design |
RC-K8 is a diffuse large B cell lymphoma cell line that responds to IL-21 stimulation with apoptosis. By chronically treating RC-K8 cells with IL-21 with increasing doses for 10 weeks we were able to generate a resistant RC-K8 cell line that will be referred to as RC-K8-R. Both RC-K8 and RC-K8-R cells were treated with IL-21 at 100ng/mL. After 6 hours of treatment cells were lysed and total mRNA was collected. Arrays were performed on Agilent Oligo microarrays. We used microarrays to detail the changes in gene expression upon Interleukin-21 treatment in diffuse large B cell lymphoma cell lines and observed distinct up-regulated and down-regulated genes. Replicates are not included. Human Reference RNA was used as reference.
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| Contributor(s) |
Sarosiek K, Malumbres R, Nechushtan H, Gentles AJ, Avisar E, Lossos I |
| Citation(s) |
19965678 |
| Submission date |
Nov 10, 2009 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Kristopher A Sarosiek |
| Organization name |
University of Miami
|
| Street address |
1580 NW 10th Ave
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| City |
Miami |
| State/province |
FL |
| ZIP/Postal code |
33138 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| GSM469547 |
RC-K8-R cells, treated with Interleukin-21 |
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| Relations |
| BioProject |
PRJNA121011 |
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