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| Status |
Public on Dec 21, 2022 |
| Title |
The EMT-regulator ZEB1 shapes bone marrow immunological niches in AML suppressing T-cell activity and fostering Th17 expansion |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
We performed gene expression profiling to evaluate potential differences between Zeb1 expressing and Zeb1 silenced cells
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| Overall design |
C1498 cells were treated overnight with lentiviral particles containing plasmid expressing a short hairpin RNA (shRNA) sequence against ZEB-1 (seq C, or seq D or a negative control construct, Scr) and green fluorescence protein (GFP). Two weeks after infection, GFP+ cells were isolated and used for RNA extraction and hybridization on microarrays.
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| Contributor(s) |
Bassani B, Simonetti G, Sangaletti S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 22, 2021 |
| Last update date |
Dec 24, 2022 |
| Contact name |
Barbara Bassani |
| E-mail(s) |
babie.bass@gmail.com
|
| Organization name |
Fondazione IRCCS Istituto Nazionale dei Tumori di Milano
|
| Department |
Department of Research
|
| Lab |
Molecular Immunology
|
| Street address |
via G. Antonio Amadeo, 42
|
| City |
Milano |
| ZIP/Postal code |
20133 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA791633 |
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