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| Status |
Public on Mar 15, 2022 |
| Title |
Maternal folic acid mild over consumption in mice leads to sex-specific transcriptional alterations in E17.5 embryonic cerebrum |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Food fortification of folic acid (FA) and increased use of vitamin supplements have raised concerns over high folate intake periconceptionally. We reported that diet containing 5-fold higher FA than recommended for mice (5xFASD) during pregnancy, resulted in neurobehavioral abnormalities (hyperactivity and short-term memory impairment) in newborns. Our goal was to determine the origins of these changes and the underlying mechanisms by examing potential transcription changes in E17.5 embryonic cerebrum.
We used microarrays to compare the E17.5 cerebral gene expression changes by 5xFASD and indentified different transcription profiles due to diet or sex.
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| Overall design |
At weaning, wild-type C57BL/6 female mice were placed on control diet (CD) or 5xFASD for a month before mating, and were maintained on the same diets until embryonic day 17.5 (E17.5). Cerebrums of both sexes from both diets were used for RNA extraction and were performed gene expression analysis by microarray using Affymetrix Clariom S array_Mouse Chip.
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| Contributor(s) |
Luan Y, Cosín-Tomás M, Leclerc D, Malysheva OV, Caudill MA, Rozen R |
| Citation(s) |
35268026 |
| Submission date |
Jan 10, 2022 |
| Last update date |
Mar 18, 2022 |
| Contact name |
Rima Rozen |
| E-mail(s) |
rima.rozen@mcgill.ca
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| Organization name |
McGill University
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| Department |
Departments of Human Genetics and Pediatrics
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| Street address |
1001 Decarie Blvd
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| City |
Montreal |
| State/province |
Quebec |
| ZIP/Postal code |
H4A 3J1 |
| Country |
Canada |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (16)
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| GSM5795851 |
male whose mother was fed CD, biological rep 1 |
| GSM5795852 |
male whose mother was fed CD, biological rep 2 |
| GSM5795853 |
male whose mother was fed CD, biological rep 3 |
| GSM5795854 |
male whose mother was fed CD, biological rep 4 |
| GSM5795855 |
male whose mother was fed FASD, biological rep 1 |
| GSM5795856 |
male whose mother was fed FASD, biological rep 2 |
| GSM5795857 |
male whose mother was fed FASD, biological rep 3 |
| GSM5795858 |
male whose mother was fed FASD, biological rep 4 |
| GSM5795859 |
female whose mother was fed CD, biological rep 1 |
| GSM5795860 |
female whose mother was fed CD, biological rep 2 |
| GSM5795861 |
female whose mother was fed CD, biological rep 3 |
| GSM5795862 |
female whose mother was fed CD, biological rep 4 |
| GSM5795863 |
female whose mother was fed FASD, biological rep 1 |
| GSM5795864 |
female whose mother was fed FASD, biological rep 2 |
| GSM5795865 |
female whose mother was fed FASD, biological rep 3 |
| GSM5795866 |
female whose mother was fed FASD, biological rep 4 |
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| Relations |
| BioProject |
PRJNA795918 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE193352_RAW.tar |
18.1 Mb |
(http)(custom) |
TAR (of CEL) |
| GSE193352_microarray_processed_results_E17.5_cerebrum.xlsx |
109.4 Kb |
(ftp)(http) |
XLSX |
| GSE193352_processed_data_matrices.xls.gz |
3.2 Mb |
(ftp)(http) |
XLS |
| Processed data are available on Series record |
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