|
| Status |
Public on Feb 16, 2023 |
| Title |
Chromatin remodeling in bovine oocytes and preimplantation embryos |
| Organism |
Bos taurus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Profiles of H3K4me3, H3K27ac, H3K27me3 and H3K9me3 in bovine GV oocytes and preimplantation embryos, and the characterization of chromatin accessibility in bovine blastocyst, inner cell mass and trophectoderm.
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| |
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| Overall design |
H3K4me3, H3K27ac, H3K27me3 and H3K9me3 were profiled with CUT&Tag technique in bovine GV oocytes (GV), 2-cell embryos (2C), 4-cell embryos (4C), 8-cell embryos (8C), morula (MO), blastocyst (BL), inner cell mass (ICM) and trophectoderm (TE). To investigate the relationship between embryonic transcription and chromatin remodeling, embryos were concurrently cultured in the presence of a transcriptional inhibitor (α-amanitin) and α-amanitin-treated 8-cell embryos (8A) were collected for library construction of H3K4me3, H3K27ac, H3K27me3 and H3K9me3. ATAC-seq libraries were prepared from bovine blastocyst, inner cell mass and trophectoderm.
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| |
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| Contributor(s) |
Zhou C, Schultz RM, Ross PJ |
| Citation(s) |
36779365 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 HD070044 |
Epigenetic control of preimplantation embryo development |
UNIVERSITY OF CALIFORNIA DAVIS |
Pablo Juan Ross |
|
| Submission date |
Jan 13, 2022 |
| Last update date |
Feb 17, 2023 |
| Contact name |
Chuan Zhou |
| E-mail(s) |
chczhou@ucdavis.edu
|
| Organization name |
University of California, Davis
|
| Department |
Animal Science
|
| Lab |
Ross Lab
|
| Street address |
Meyer Hall, 450 Bioletti Way
|
| City |
Davis |
| State/province |
CA |
| ZIP/Postal code |
95616 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
|
| Samples (81)
|
|
| Relations |
| BioProject |
PRJNA797029 |
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