|
| Status |
Public on Jan 27, 2010 |
| Title |
Genome wide mapping of Jarid2 and Suz12 binding sites in mouse ES cells before and after Jarid2 depletion |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We analyzed the overlap in genome wide binding sites between Jarid2 and Suz12 in mouse ES cells and find that Jarid2 and Suz12 peaks have an high degree (90%) of overlap. Moreover we analyzed the effect of Jarid2 down regulation on genome wide Suz12 binding sites and found that Loss of Jarid2 lead to the loss of 70% of Suz12 binding sites and to a 10 Fold reduction in intensity for 90% of Suz12 binding sites. Overall, these results demonstrate that Jarid2 plays an essential role for Suz12 (PRC2 complex) association to DNA.
|
| |
|
| Overall design |
Examination of two different proteins in two different cell lines
|
| |
|
| Contributor(s) |
Pasini D, Bak M, Helin K |
| Citation(s) |
20075857 |
| Submission date |
Dec 07, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Diego Pasini |
| E-mail(s) |
diego.pasini@ieo.it
|
| Organization name |
European Institute of Oncology
|
| Department |
Experimental Oncology
|
| Street address |
Via Adamello, 16
|
| City |
Milano |
| ZIP/Postal code |
20139 |
| Country |
Italy |
| |
|
| Platforms (1) |
| GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| SRA |
SRP001801 |
| BioProject |
PRJNA120403 |
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