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| Status |
Public on Dec 01, 2022 |
| Title |
Next generation sequencing of chicken otocyst with gain- and loss-of-Notch signalling activity |
| Organism |
Gallus gallus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Bulk RNA-sequencing of individual chicken otocysts, in which Notch activity was either stimulated or blocked. To stimulate Notch activity, the E2 otic placodes were in ovo transfected with a construct overexpressing Notch1 Intracellular domain (NICD1) or a control plasmid expressing mRFP1 and collected 6h or 24h later. To pharmacologically block Notch activity, E2.5 chicken otocysts were incubated in media enriched with γ-secretase
Inhibitor or control condition (DMSO) for 6h or 24h. Transcripts abundance was mapped to a chicken reference genome using Kallisto package.
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| |
|
| Overall design |
Identification of genes deregulated in the otocyst in response to the manipulation of Notch signaling activity
|
| |
|
| Contributor(s) |
Żak M, Daudet N |
| Citation(s) |
37900277 |
| Submission date |
Feb 18, 2022 |
| Last update date |
Nov 13, 2023 |
| Contact name |
Magdalena Zak |
| E-mail(s) |
m.zak@ucl.ac.uk
|
| Organization name |
UCL Ear Institute
|
| Street address |
332 Grays Inn Road
|
| City |
London |
| ZIP/Postal code |
WC1X 8EE |
| Country |
United Kingdom |
| |
|
| Platforms (1) |
| GPL19787 |
Illumina NextSeq 500 (Gallus gallus) |
|
| Samples (30)
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|
| Relations |
| BioProject |
PRJNA808316 |
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