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| Status |
Public on Jul 07, 2022 |
| Title |
BMSC-derived exosomal miR-27a-3p and miR-196b-5p regulate bone remodeling in ovariectomized rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
BMSC-derived exosomes from ovariectomized rats (OVX-Exo) and sham-operated rats (Sham-Exo) were co-cultured with bone marrow-derived macrophages to study their effects on osteoclast differentiation. Next-generation sequencing was utilized to identify the differentially expressed miRNAs (DE-miRNAs) in OVX-Exo and Sham-Exo, while target genes were analyzed using bioinformatics. The regulatory effects of miR-27a-3p and miR-196b-5p on osteogenic differentiation of BMSCs and osteoclast differentiation were verified by gain-of-function and loss-of-function analyses.Osteoclast differentiation was significantly enhanced in the OVX-Exo treatment group compared to the Sham-Exo group. Twenty DE-miRNAs were identified in OVX-Exo and Sham-Exo, among which miR-27a-3p and miR-196b-5p promoted the expressions of osteogenic genes in BMSCs. In contrast, knockdown of miR-27a-3p and miR-196b-5p increased the expressions of osteoclastic genes in osteoclasts. These 20 DE-miRNAs were found to target 11435 mRNAs. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed that these target genes were involved in several biological processes and osteoporosis-related signaling pathways.
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| Overall design |
MiRNA profiles of BMSC-derived exosomes from ovariectomized rats and sham-operated rats
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| Contributor(s) |
Sang H, Lai G |
| Citation missing |
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| Submission date |
Feb 21, 2022 |
| Last update date |
Jul 07, 2022 |
| Contact name |
Guohua Lai |
| E-mail(s) |
guohualai@yeah.net
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| Organization name |
Shenzhen Hospital,Southern Medical University
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| Street address |
NO.1333,Xinhu Road,Baoan District
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| City |
Shenzhen |
| State/province |
Guangdong |
| ZIP/Postal code |
518110 |
| Country |
China |
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| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA808900 |
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