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| Status |
Public on Mar 03, 2022 |
| Title |
The transcription factor, α1ACT, acts through a microRNA network to regulate neurogenesis and cell death during neonatal cerebellar development |
| Organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
To investigate whether α1ACT acts through a microRNA network, we studied α1ACT-associated simultaneous miRNA:mRNA expression transcriptome profiles during cerebellar development in a context-dependent manner, using miRNA-seq paired with previously reported RNA-seq.
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| Overall design |
Examination the miRNA-seq results between pc12 cell lines stably expressing blank vector and α1ACT; pairing the differentially expressed miRNA with previously reported DEGs via miRWALK to investigate the α1ACT mediated miRNA regulation network. All outcomes were validated via qRT-PCR
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| Contributor(s) |
Wei C, Du X |
| Citation missing |
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| Submission date |
Mar 01, 2022 |
| Last update date |
Mar 03, 2022 |
| Contact name |
Christopher Gomez |
| E-mail(s) |
cgomez@neurology.bsd.uchicago.edu
|
| Phone |
7737026047
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| Organization name |
University of Chicago
|
| Department |
Neurology
|
| Street address |
5812 S Ellis Ave Room J219
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| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL14844 |
Illumina HiSeq 2000 (Rattus norvegicus) |
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| Samples (23)
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| Relations |
| BioProject |
PRJNA811520 |
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