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| Status |
Public on Feb 28, 2023 |
| Title |
Simultaneous single-cell analysis of 5mC and 5hmC with SIMPLE-seq |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We used a new technology, named SIMPLE-seq, to perform simultaneous profiling of 5mC and 5hmC on mESCs and PBMCs at single-cell,single molecule level.
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| Overall design |
We develop SIMPLE-seq for simultaneously profiling the genome-wide true methylome and hydroxymethylome quantitatively, in highly scalable single-cell manner. SIMPLE-seq measures both true 5mC and 5hmC on the same DNA molecule from one cell. To demonstrate the utility of SIMPLE-seq, we measured single-cell methylome and hydroxymethylome in mouse embryonic stem cells (mESC) and human peripheral blood mononuclear cells.
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| Contributor(s) |
Bai D, Zhu C |
| Citation(s) |
38336903 |
| Submission date |
Mar 02, 2022 |
| Last update date |
Apr 10, 2024 |
| Contact name |
Dongsheng Bai |
| E-mail(s) |
baidongsheng@pku.edu.cn
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| Phone |
13261502638
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| Organization name |
Peking University
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| Department |
School of Life Sciences
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| Lab |
Chengqi Yi
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| Street address |
Yiheyuan Road,No 5
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100871 |
| Country |
China |
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| Platforms (3) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA811798 |
