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| Status |
Public on Jun 16, 2022 |
| Title |
Sex-specific modulation of the host transcriptome in the spleen of Schistosoma mansoni infected mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Intestinal Schistosomiasis, caused by the helminth Schistosoma mansoni, results in fibroproliferative disease triggered by liver trapped parasite eggs. Characteristic of the infection is a chronic course of disease with progressive periportal fibrosis, spleno- and hepatomegaly, and portal hypertension. Despite significant progress in understanding the complex immunological processes involved in the host-pathogen relationship, there is currently no effective vaccine against the infection nor antifibrotic drugs, increasing the pressure to find new targets for schistosome drugs and vaccine candidates. We have recently reported that infection with male schistosomes can attenuate reinfections, while female schistosomes appear to weaken the host immune system. To decipher the underlying mechanisms of differential immunogenicity of female and male worms, we performed a comparative transcriptomic analysis of splenic tissue from unisexually and bisexually infected mice. We identified a total of 1293, 512 and 4062 genes, respectively, that were differentially expressed in the group of male-only (M), female-only (F) or bisexually (MF) infected mice compared to naive controls. Bisexual infection and infection with male worms showed the marked effects on host immune response, erythrocyte homeostasis, lipoprotein metabolism and cell cycle processes compared to the infection with female worms. Furthermore, we could show that both worm sexes trigger immune responses in an egg-independent manner, with a non-polarized Th1 and Th2 response, with female worms having a lower regulatory influence than males.
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| Overall design |
Six-week-old female C57BL/6 mice were percutaneously exposed to 100 S. mansoni cercariae, either male only in 3 mice, female only in three mice, or both sexes (50 + 50 cercariae) in 2 mice. Controls were left untreated in 2 mice.
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| Contributor(s) |
Winkelmann F, Rabes A, Schulz C, Koslowski N, Koczan D, Reisinger EC, Sombetzki M |
| Citation(s) |
35865813 |
| Submission date |
Mar 02, 2022 |
| Last update date |
Aug 03, 2022 |
| Contact name |
Dirk Koczan |
| E-mail(s) |
dirk.koczan@med.uni-rostock.de
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| Phone |
+493814945885
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| Organization name |
University of Rostock
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| Department |
Institute for Immunology
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| Street address |
Schillingallee 70
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| City |
Rostock |
| ZIP/Postal code |
18057 |
| Country |
Germany |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA812064 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE197804_RAW.tar |
13.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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