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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 04, 2022 |
| Title |
RNA sequencing of HUVECs stimulated with VEGF, OSM+VEGF, or CNTF+CNTFR+VEGF with or without STAT3 knock-down |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The role of STAT3 signaling induced by stimulation of IL-6 cytokine family members such as OSM and CNTF on the angiogenic response of endothelial cells is still not fully understood. To assess the impact of this pathway on angiogenesis, this study compares human umbilical vein endothelial cells (HUVECs) with or without STAT3 knock-down introduced by siRNA and stimulation with VEGF, OSM+VEGF and CNTF+VEGF in the presence of the CNTF receptor (CNTFR).
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| Overall design |
mRNA profiles of HUVECs stimulated with VEGF (n=4), OSM+VEGF (n=4), or CNTF+CNTFR+VEGF (n=4) for 24h transfected with control siRNA. The samples were compared to HUVECs transfected with STAT3 siRNA and incubated by the same cytokines for 24 (n=4 each).
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| Contributor(s) |
Rapp J, Bucher F |
| Citation(s) |
36458801 |
| Submission date |
Mar 11, 2022 |
| Last update date |
Dec 06, 2022 |
| Contact name |
Julian Rapp |
| E-mail(s) |
julian.rapp.work@gmail.com
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| Organization name |
University Medical Center Freiburg
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| Street address |
Killianstraße 5
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| City |
Freiburg im Breisgau |
| ZIP/Postal code |
79106 |
| Country |
Germany |
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| Platforms (1) |
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| Samples (24)
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| GSM5949388 |
HUVEC, VEGF, Control siRNA, rep4 |
| GSM5949389 |
HUVEC, OSM_VEGF, Control siRNA, rep1 |
| GSM5949390 |
HUVEC, OSM_VEGF, Control siRNA, rep2 |
| GSM5949391 |
HUVEC, OSM_VEGF, Control siRNA, rep3 |
| GSM5949392 |
HUVEC, OSM_VEGF, Control siRNA, rep4 |
| GSM5949393 |
HUVEC, CNTF_R_VEGF, Control siRNA, rep1 |
| GSM5949394 |
HUVEC, CNTF_R_VEGF, Control siRNA, rep2 |
| GSM5949395 |
HUVEC, CNTF_R_VEGF, Control siRNA, rep3 |
| GSM5949396 |
HUVEC, CNTF_R_VEGF, Control siRNA, rep4 |
| GSM5949397 |
HUVEC, VEGF, STAT3 siRNA, rep1 |
| GSM5949398 |
HUVEC, VEGF, STAT3 siRNA, rep2 |
| GSM5949399 |
HUVEC, VEGF, STAT3 siRNA, rep3 |
| GSM5949400 |
HUVEC, VEGF, STAT3 siRNA, rep4 |
| GSM5949401 |
HUVEC, OSM_VEGF, STAT3 siRNA, rep1 |
| GSM5949402 |
HUVEC, OSM_VEGF, STAT3 siRNA, rep2 |
| GSM5949403 |
HUVEC, OSM_VEGF, STAT3 siRNA, rep3 |
| GSM5949404 |
HUVEC, OSM_VEGF, STAT3 siRNA, rep4 |
| GSM5949405 |
HUVEC, CNTF_R_VEGF, STAT3 siRNA, rep1 |
| GSM5949406 |
HUVEC, CNTF_R_VEGF, STAT3 siRNA, rep2 |
| GSM5949407 |
HUVEC, CNTF_R_VEGF, STAT3 siRNA, rep3 |
| GSM5949408 |
HUVEC, CNTF_R_VEGF, STAT3 siRNA, rep4 |
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| Relations |
| BioProject |
PRJNA815366 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE198484_RAW.tar |
7.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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