 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Apr 03, 2022 |
| Title |
Spatiotemporal expression of HMGB2 regulates cell proliferation and hepatocyte size during liver regeneration |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Liver regeneration is an extraordinarily complex process involving a variety of factors; however, the role of chromatin protein in hepatocyte proliferation is largely unknown. In this study, we investigated the functional role of high-mobility group box 2 (HMGB2), a chromatin protein in liver regeneration using wild-type and HMGB2-knockout (KO) mice. Liver tissues were sampled after 70% partial hepatectomy (PHx), and analyzed by immunohistochemistry using various markers of cell proliferation, including Ki-67, PCNA, cyclin D1, cyclin B1, EdU and pH3S10. In WT mice, hepatocyte proliferation was strongly correlated with the spatiotemporal expression of HMGB2; however, cell proliferation was significantly delayed in hepatocytes of HMGB2-KO mice. Quantitative PCR demonstrated that cyclin D1 and cyclin B1 mRNAs were significantly decreased in HMGB2-KO mice livers. Interestingly, hepatocyte size was significantly larger in HMGB2-KO mice at 36-72 h after PHx, and these results suggest that hepatocyte hypertrophy appeared in parallel with delayed cell proliferation. In vitro experiments demonstrated that cell proliferation was significantly decreased in HMGB2-KO cells. A significant delay in cell proliferation was also found in HMGB2-siRNA transfected cells. In summary, spatiotemporal expression of HMGB2 is important for regulation of hepatocyte proliferation and cell size during liver regeneration.
|
| |
|
| Overall design |
Microarray analysis was used to screen for genes that are related to HMGB2 expression. RNA was isolated from WT and HMGB2-KO mouse livers (n = 3 each), and cRNA was prepared. cRNA from each sample was hybridized to a pre-equilibrated Clariom S mouse microarray chip, which was then washed, stained, and scanned in an HP ChipScanner (Affymetrix Inc., Santa Clara, USA).
|
| |
|
| Contributor(s) |
Choijookhuu N, Yano K |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 01, 2022 |
| Last update date |
Jul 14, 2022 |
| Contact name |
Koichi Yano |
| E-mail(s) |
k01yano@med.miyazaki-u.ac.jp
|
| Organization name |
University of Miyazaki
|
| Department |
Department of Anatomy, Histochemistry and Cell Biology, Faculty of Medicine
|
| Street address |
5200 Kihara, Kiyotake
|
| City |
Miyazaki |
| State/province |
Miyazaki |
| ZIP/Postal code |
889-1692 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA822301 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE199944_RAW.tar |
6.2 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...