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| Status |
Public on Apr 01, 2024 |
| Title |
Effects of KRAS (G13C) expression on transcriptome profiles assessed in HPCs differentiated from patient-derived iPS cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
How a single oncogenic RAS impacts non-transformed hematopoietic progenitor cells (HPCs) remains largely unknown. We therefore investigated how a monoallelic KRAS mutation (G13C) affected HPCs utilizing induced pluripotent stem cells (iPSCs) derived from two unrelated patients with RAS-associated autoimmune lymphoproliferative syndrome-like disease (RALD).
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| Overall design |
We performed gene expression profiling analysis of 12 HPC samples in total, with 6 samples being from each patient. The two clones from each patient constituted an isogenic pair of different genotypes (WT/WT and WT/G13C). Three samples obtained from each clone were subjected to the analysis, one with no stimulation, and the other two each stimulated with different cytokine combinations.
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| Contributor(s) |
Lin H, Takagi M, Kubara K, Yamazaki K, Michikawa F, Okumura T, Naruto T, Morio T, Miyazaki K, Taniguchi H, Otsu M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 11, 2022 |
| Last update date |
Apr 01, 2024 |
| Contact name |
Makoto Otsu |
| E-mail(s) |
motsu@med.kitasato-u.ac.jp
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| Organization name |
Kitasato University School of Medicine
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| Street address |
1-15-1 Kitasato, Minami-ku
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| City |
Sagamihara |
| State/province |
Kanagawa |
| ZIP/Postal code |
252-0374 |
| Country |
Japan |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (12)
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| GSM6038480 |
Pt1 iPSC-HPCs, WTcontrol, unstimulated |
| GSM6038481 |
Pt1 iPSC-HPCs, WTcontrol, stimulated_SF |
| GSM6038482 |
Pt1 iPSC-HPCs, WTcontrol, stimulated_STF3 |
| GSM6038483 |
Pt1 iPSC-HPCs, KRASG13C, unstimulated |
| GSM6038484 |
Pt1 iPSC-HPCs, KRASG13C, stimulated_SF |
| GSM6038485 |
Pt1 iPSC-HPCs, KRASG13C, stimulated_STF3 |
| GSM6038486 |
Pt2 iPSC-HPCs, WTcontrol, unstimulated |
| GSM6038487 |
Pt2 iPSC-HPCs, WTcontrol, stimulated_SF |
| GSM6038488 |
Pt2 iPSC-HPCs, WTcontrol, stimulated_STF3 |
| GSM6038489 |
Pt2 iPSC-HPCs, KRASG13C, unstimulated |
| GSM6038490 |
Pt2 iPSC-HPCs, KRASG13C, stimulated_SF |
| GSM6038491 |
Pt2 iPSC-HPCs, KRASG13C, stimulated_STF3 |
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| Relations |
| BioProject |
PRJNA825504 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE200600_RAW.tar |
81.7 Mb |
(http)(custom) |
TAR (of XLSX) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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