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Series GSE200619 Query DataSets for GSE200619
Status Public on Apr 01, 2024
Title Real-time quantitative PCR analysis of mice kidney after administration of Lu-177-octreotate, Lu-177-octreotate + A1M and A1M
Organism Mus musculus
Experiment type Expression profiling by RT-PCR
Summary This work aimed to examine the regulation of apoptosis-related genes in kidney cortex and kindey medulla one and seven days after administration of 177Lu-octreotate with and without A1M and of A1M alone. C57BL/6N mice were injected with either 177Lu-octreotate + PBS, A1M + PBS or 177Lu-octreotate + A1M. Also, a control group was sham-treated with saline. Half of the animals in each treatment and control group were terminated by cardiac puncture one-day post-injection (1 dpi), and the remaining animals were terminated at 7dpi. Kidneys were dissected at the time of termination, snap-frozen in liquid nitrogen and stored at -80°C.
 
Overall design Three biological replicated were included in each test group at both 1 dpi and 7dpi, and three biological replicates were included in corresponding controls. An equal amount of total RNA (40 µg) was extracted from all samples, and 200 ng of total RNA was reversed transcribed into cDNA per sample.
 
Contributor(s) Andersson C, Simonsson K, Shubbar E, Gram M, Helou K, Forssell-Aronsson E
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Submission date Apr 11, 2022
Last update date Apr 01, 2024
Contact name Charlotte Andersson
E-mail(s) charlotte.andersson@gu.se
Organization name University of Gothenburg
Department Department of Medical Radiation Science
Lab Forssell-Aronsson Lab
Street address Gula Stråket 2B
City Gothenburg
ZIP/Postal code 413 45
Country Sweden
 
Platforms (1)
GPL32158 QIAGEN RT² Profiler™PCR Array Mouse Apoptosis
Samples (60)
GSM6039070 Medulla_150MBq_1dpi_rep1 [SAMPLE 1]
GSM6039071 Medulla_150MBq_1dpi_rep2 [SAMPLE 2]
GSM6039072 Medulla_150MBq_1dpi_rep3 [SAMPLE 3]
Relations
BioProject PRJNA825545

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE200619_data_sheets.xlsx 199.1 Kb (ftp)(http) XLSX
Processed data included within Sample table
Processed data are available on Series record

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