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| Status |
Public on Jun 17, 2022 |
| Title |
Gene expression profiling of melanoma cell lines with acquired resistance to the BRAF inhibitor PLX4032 and the parental sensitive counterparts and of melanoma cell lines intrinsically resistant. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Targeted therapy with small molecules inhibiting BRAF and MEK kinase represents a therapeutic option in advanced metastatic melanoma although drug resistance limits the achievement of persistent cures. The genomic analyses of tumors from patients developing treatment resistance indicate that drug tolerance may result from non-mutational events occurring in the tumor microenvironment. To identify tumor specific transcriptional changes related to resistance to the BRAF-inhibitor PLX4032, cell lines with acquired resistance to PLX4032 were generated from six sensitive melanoma lines by chronic in vitro drug exposure (Vergani E, Oncotarget 2016) and analyzed for gene expression profiles; in addition a set of cell lines with intrinsic resistance was included for comparison. Cell lines were obtained from metastatic surgical specimens and were previously genetically characterized (Daniotti M, Oncogene 2004; Vergani E, JID 2022, accepted).
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| Overall design |
Gene expression profiling of six pairs of Resistant (Ra)/Sensitive (S) melanoma cell lines, and of a set of cell lines with intrinsic resistance (Ri) was performed by Clariom S platform.
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| Contributor(s) |
De Cecco L, Vergani E |
| Citation(s) |
35912092 |
| Submission date |
Apr 29, 2022 |
| Last update date |
Aug 03, 2022 |
| Contact name |
Loris De Cecco |
| E-mail(s) |
loris.dececco@istitutotumori.mi.it
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| Organization name |
IRCSS Istituto Nazionale Tumori
|
| Street address |
via Venezian 1
|
| City |
Milan |
| ZIP/Postal code |
20133 |
| Country |
Italy |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA833454 |
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