|
| Status |
Public on Apr 29, 2024 |
| Title |
RNA sequencing of gastric organoids treated with bacteria |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We treated mouse gastric organoids with Helicobacter pylori, Fusobacterium nucleatum, and Neisseria subflava, and extracted RNA after 4 hours. RNAs were applied to RNA sequencing analysis to investigate bacteria-specific effects on gastric epithelial cells.
|
| |
|
| Overall design |
Examination of transcriptomic analysis on bacteria-treated gastric organoids.
|
| |
|
| Contributor(s) |
Hayakawa Y, Niikura R, Nagata N, Fujishiro M |
| Citation |
Non-*Helicobacter pylori* Gastric Microbiome Modulates Prooncogenic Responses and Is Associated With Gastric Cancer Risk https://doi.org/10.1016/j.gastha.2023.03.010
|
| Submission date |
May 09, 2022 |
| Last update date |
May 03, 2024 |
| Contact name |
Yoku Hayakawa |
| E-mail(s) |
hayakawayoku@gmail.com, yhayakawa-tky@umin.ac.jp
|
| Phone |
81-3-38155411
|
| Organization name |
University of Tokyo
|
| Street address |
7-3-1, Hongo, Bunkyo-ku, Clinical Research Center 4F, Room 425
|
| City |
Tokyo |
| State/province |
TOKYO |
| ZIP/Postal code |
1138655 |
| Country |
Japan |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (12)
|
| GSM6123315 |
Fusobacterium-treated gastric organoids [Fn-1] |
| GSM6123316 |
Fusobacterium-treated gastric organoids [Fn-2] |
| GSM6123317 |
Fusobacterium-treated gastric organoids [Fn-3] |
| GSM6123318 |
Helicobacter-treated gastric organoids [Hp-1] |
| GSM6123319 |
Helicobacter-treated gastric organoids [Hp-2] |
| GSM6123320 |
Helicobacter-treated gastric organoids [Hp-3] |
| GSM6123321 |
Neisseria-treated gastric organoids [Ns-1] |
| GSM6123322 |
Neisseria-treated gastric organoids [Ns-2] |
| GSM6123323 |
Neisseria-treated gastric organoids [Ns-3] |
| GSM6123324 |
untreated control gastric organoids [organoid-1] |
| GSM6123325 |
untreated control gastric organoids [organoid-2] |
| GSM6123326 |
untreated controlgastric organoids [organoid-3] |
|
| Relations |
| BioProject |
PRJNA836409 |
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