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| Status |
Public on Mar 01, 2010 |
| Title |
Molecular analysis of endothelial progenitor cell (EPC) subtypes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Genome -wide analysis of EPC subtypes obtained from both umbilical cord and peripheral blood. This experiment sought to compare the expression levels of eEPCs and OECs against monocytes and human dermal microvascular cells (HDMECs) in order to establish if these cells are linked to a monocytic or endothelial lineage. This is very important as the term EPC is currently used to describe cell populations which are quite dissimilar in terms of biological properties.
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| Overall design |
Total RNA was isolated from 7 day old eEPCs, early passage OECs, freshly isolated CD14+ monocytes and early passage DMECs using RNAqueous kit. Three biological replicates per EPC subtype from peripheral blood was used for trancriptome analysis using Illumina Human WG-6 v3.9 beadchip.
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| Contributor(s) |
Medina RJ, O'Neill CL, Sweeney M, Guduric-Fuchs J, Gardiner TA, Simpson DA, Stitt AW |
| Citation(s) |
20465783 |
| Submission date |
Feb 11, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Christina L O'Neill |
| E-mail(s) |
coneill55@qub.ac.uk
|
| Organization name |
Queens University Belfast
|
| Department |
Centre for Vision and Vascular Science
|
| Street address |
Centre for Vision & Vascular Science Institute of Clinical Sciences A Grosvenor Road Royal Victoria Hospital
|
| City |
Belfast |
| State/province |
Northern Ireland |
| ZIP/Postal code |
BT12 6BA |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA125315 |
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