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Series GSE2050 Query DataSets for GSE2050
Status Public on Dec 09, 2004
Title Major Patterns of B Cell Gene Expression Changes in Response to Short-Term Stimulation with 33 Single Ligands
Organism Mus musculus
Experiment type Expression profiling by array
Summary We examined the major patterns of changes in gene expression in mouse splenic B cells in response to stimulation with 33 single ligands for 0.5, 1, 2, and 4 h. We found that ligands known to directly induce or costimulate proliferation, namely, anti-IgM (anti-Ig), anti-CD40 (CD40L), LPS, and, to a lesser extent, IL-4 and CpG-oligodeoxynucleotide (CpG), induced significant expression changes in a large number of genes. The remaining 28 single ligands produced changes in relatively few genes, even though they elicited measurable elevations in intracellular Ca2+ and cAMP concentration and/or protein phosphorylation, including cytokines, chemokines, and other ligands that interact with G protein-coupled receptors. A detailed comparison of gene expression responses to anti-Ig, CD40L, LPS, IL-4, and CpG indicates that while many genes had similar temporal patterns of change in expression in response to these ligands, subsets of genes showed unique expression patterns in response to IL-4, anti-Ig, and CD40L.
Keywords: other
 
 
Contributor(s) Hart R, Chang M, Kim J, Cao Y, Lee S, Alexander A, Grossoehme J, Yan Z, Hsueh R, Choi S
Citation(s) 15585835
Submission date Dec 08, 2004
Last update date Mar 16, 2012
Contact name Sangdun Choi
E-mail(s) schoi@its.caltech.edu
Phone 626-395-8732
URL http://www.signaling-gateway.org
Organization name California Institute of Technology
Department Biology
Lab Alliance for Cellular Signaling Molecular Biology Laboratory
Street address 1200 E. California Blvd
City Pasadena
State/province CA
ZIP/Postal code 91125
Country USA
 
Platforms (1)
GPL260 Caltech 16K cDNA Mouse Array
Samples (422)
GSM5420 Spleen vs 2-Methyl-thio-ATP Treated B cell 30min (set1)
GSM5422 Spleen vs 2-Methyl-thio-ATP Treated B cell 30min (set2)
GSM5423 Spleen vs 2-Methyl-thio-ATP Treated B cell 30min (set3)
Relations
BioProject PRJNA91707

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Supplementary data files not provided
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