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| Status |
Public on Jun 07, 2022 |
| Title |
Influence of sire fertility status on conceptus-induced transcriptomic response of the bovine endometrium |
| Organism |
Bos taurus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The aim of this study was to examine the effect of sire fertility status on conceptus-induced changes in the endometrial transcriptome. Holstein Friesian bulls (3 High fertility, HF, 3 Low fertility, LF) were selected from the Irish national population of AI bulls (minimum of 500 inseminations/bull) based on adjusted fertility scores (HF: +4.37% and LF: -12.7%; mean = 0%). To generate elongated conceptuses, Day 7 blastocysts produced in vitro using sperm from these six bulls were transferred in groups of 5-10 to synchronized heifers (n=7 heifers per bull; total 42 heifers). Conceptuses were recovered following slaughter on Day 15 (recovery rate: HF 59.4% vs. LF 45.0%; P<0.05). In parallel, Day 15 endometrial explants were recovered from synchronized cyclic heifers (n=4). Explants from each heifer were co-cultured for 6 h in RPMI medium with (i) nothing, control (ii) 100 ng/ml ovine recombinant interferon tau (IFNT) (iii) a single conceptus from each high fertility bull, or (iv) a single conceptus from each low fertility bull. To minimize variation, explants from the same uterus were used across all treatments, replicated across 4 heifers. After 6 h, explants were snap frozen and stored at -80°C. Extracted mRNA was subjected to RNA-seq (Illumina NextSeq 500) and the resulting data were analyzed through a bioinformatic pipeline with R software.
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| Overall design |
Endometrial explants were co-cultured for 6 h in RPMI medium with (i) nothing, control (ii) 100 ng/ml ovine recombinant interferon tau (IFNT) (iii) a single conceptus from each high fertility bull, or (iv) a single conceptus from each low fertility bull.
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| Contributor(s) |
O’Callaghan E, Sánchez JM, Rabaglino MB, McDonald M, Liu H, Spencer TE, Fair S, Kenny DA, Lonergan P |
| Citation(s) |
36072344 |
| Submission date |
Jun 02, 2022 |
| Last update date |
Sep 16, 2022 |
| Contact name |
Hongyu Liu |
| E-mail(s) |
hliu5259@gmail.com
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| Phone |
5714744017
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| Organization name |
university of missouri
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| Street address |
920 east campus drive.
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| City |
columbia |
| State/province |
mo |
| ZIP/Postal code |
65211 |
| Country |
USA |
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| Platforms (1) |
| GPL23055 |
Illumina NextSeq 500 (Bos taurus) |
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| Samples (32)
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| Relations |
| BioProject |
PRJNA844972 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE205404_gene_count_matrix.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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