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| Status |
Public on Mar 08, 2011 |
| Title |
Gene expression in human umbilical cord endothelial cells following premiR-100 overexpression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
To search for genes regulated by microRNA-100 in endothelial cells, we transfected human umbilical cord endothelial cells (HUVECs) with miR-100 precursor oligonucelotides. Human umbilical vein endothelial cells (HUVECs) were isolated from donated umbilical cords, pooled from two donors and cultivated up to passage 5. For transfection with pre-miR microRNA precursor molecules cells were cultured to 70% confluence and transfected with 8nM pre-miR-100 or an irrelevant control oligonucleotide (both from Ambion) using Lipofectamin RNAiMax (Invitrogen) according to the manufacturers instructions. Total RNA was isolated 48h after transfection.
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| |
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| Overall design |
Two individual samples per condition were analyzed.
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| Contributor(s) |
Grundmann S, Moser M |
| Citation(s) |
21339483 |
| Submission date |
Mar 08, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Sebastian Grundmann |
| E-mail(s) |
sebastian.grundmann@uniklinik-freiburg.de
|
| Organization name |
University Hospital Freiburg
|
| Street address |
Hugstetter Str. 55
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA124925 |
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