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| Status |
Public on Oct 04, 2010 |
| Title |
Expression data from WT and TCF-1-deficient memory CD8 T cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
TCF-1 is an HMG family transcription factor which is known to be activated by the canonical Wnt signaling pathway and modulated by other signals such as those derived from T cell receptor. We found that during CD8 T cell responses, TCF-1 deficiency impaired long-term maintenance of antigen-specific memory CD8 T cells.
We used microarrays to detect gene expression changes in memory CD8 T cells caused by TCF-1 deficiency.
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| Overall design |
Host mice that received WT or TCF-1-deficient OT-I CD8 T cells were infected with attenuated Listeria monocytogenes expressing Ova peptide. In memory phase (i.e., more than 80 days post infection), antigen-specific T cells were ioslated by cell sorting. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).
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| Contributor(s) |
Xue H, Zhou X |
| Citation(s) |
20727791 |
| Submission date |
Mar 11, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Hai-Hui Xue |
| E-mail(s) |
haihui.xue@hmh-cdi.org
|
| Organization name |
Hackensack University Medical Center
|
| Department |
Center for Discovery and Innovation
|
| Street address |
111 Ideation Way
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| GSM520717 |
WT CD8 memory T cells, biological rep1 |
| GSM520718 |
WT CD8 memory T cells, biological rep2 |
| GSM520719 |
WT CD8 memory T cells, biological rep3 |
| GSM520720 |
TCF-1 KO CD8 memory T cells, biological rep1 |
| GSM520721 |
TCF-1 KO CD8 memory T cells, biological rep2 |
| GSM520722 |
TCF-1 KO CD8 memory T cells, biological rep3 |
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| Relations |
| BioProject |
PRJNA125039 |
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