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| Status |
Public on Oct 06, 2022 |
| Title |
CRAC of yeast RNA polymerase II in WT and isw1Δ cells in standard conditions or upon ER stress. |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We obtained RNA polymerase II occupancy profile across the genome of WT and isw1Δ Saccharomyces cerevisiae strains grown under normal conditions or after ER stress induction for 2H with1 µg/mL tunicamycin (Tm).
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| Overall design |
The nascent RNA was crosslinked to RNAPII (Rpb1) and sequenced after purification of Rpb1 under stringent and denaturing conditions. This reveals the position of the polymerase at the nucleotide resolution level.
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| Contributor(s) |
Matabishi-Bibi L, Challal D, Barucco M, Libri D, Babour A |
| Citation(s) |
36284099 |
| Submission date |
Jul 07, 2022 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Domenico Libri |
| E-mail(s) |
domenico.libri@igmm.cnrs.fr
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| Organization name |
IGMM - CNRS
|
| Lab |
Institut Genetique de Montpellier
|
| Street address |
1919 route de Mende
|
| City |
Montpellier |
| ZIP/Postal code |
34293 CEDEX 05 |
| Country |
France |
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| Platforms (1) |
| GPL13821 |
Illumina HiSeq 2000 (Saccharomyces cerevisiae) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA856628 |
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