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| Status |
Public on Dec 24, 2023 |
| Title |
Transcriptional effects of SARS-CoV-2 on human M-CSF-derived macrophages |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Analysis of the transcriptional signature of SARS-CoV-2-infected human M-CSF-dependent monocyte-derived macrophages. Methods: Human Peripheral Blood Mononuclear Cells (PBMC) were isolated from buffy coats from donors over a Lymphoprep gradient according to standard procedures. Monocytes were purified from PBMC by magnetic cell sorting using anti-CD14 microbeads (>95% CD14+ cells). Monocytes from three independent donors (0.5 x 106 cells/ml, >95% CD14+ cells) were cultured in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) at 37°C in a humidified atmosphere with 5% CO2 and 21% O2 for 7 days in the presence of 1000 U/ml M-CSF, with cytokine addition every two days. Then, monocyte-derived macrophages were exposed SARS-CoV-2 (SARS-CoV-2 clinical isolate Gisaid EPI_ISL_1120962, corresponding to ancestral S D614G, at an MOI=1) or nothing (UNT). After 4,12 and 36 hours, cells were lysed and RNA isolated for transcriptional analysis.
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| |
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| Overall design |
mRNA profiles of human M-CSF-derived macrophages infected with SARS-CoV-2
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| |
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| Contributor(s) |
Domínguez Soto Á, Colmenares M, Vega MA, Corbí AL |
| Citation(s) |
37917179 |
| Submission date |
Jul 09, 2022 |
| Last update date |
Feb 08, 2024 |
| Contact name |
Angeles Domínguez Soto |
| E-mail(s) |
ads@cib.csic.es
|
| Organization name |
CSIC
|
| Street address |
Ramiro de Maeztu
|
| City |
Madrid |
| ZIP/Postal code |
28040 |
| Country |
Spain |
| |
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| Platforms (1) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA857312 |
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