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| Status |
Public on May 24, 2024 |
| Title |
Identify H3K4Me3 occupancy on genome in Setd1a Knock out (KO) mouse undifferentiated pleomorphic sarcoma cells |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
As Setd1a is a methyltransferase targeting H3K4, we performed H3K4Me3 ChIP in control and Setd1a KO cell to observe the effects of Setd1a on H3K4Me3.
Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H3K4Me in control mouse UPS and Setd1a KO mouse UPS cells .
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| Overall design |
Ga I Ban
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| Contributor(s) |
Ou J, Alman B |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 18, 2022 |
| Last update date |
May 24, 2024 |
| Contact name |
jianhong ou |
| E-mail(s) |
jianhong.ou@duke.edu
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| Phone |
5084102796
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| Organization name |
Duke University
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| Department |
Cell Biology
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| Lab |
Regeneromics
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| Street address |
465 Nanaline Duke Building, Duke University Medical Center
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| City |
Durham |
| State/province |
North Carolina |
| ZIP/Postal code |
27710 |
| Country |
USA |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (10)
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| GSM6341914 |
Metastatic mouse UPS cell, H3K4Me3, rep1 |
| GSM6341915 |
Metastatic mouse UPS cell, H3K4Me3, rep2 |
| GSM6341916 |
Metastatic mouse UPS cell lacking Setd1a, H3K4Me3, rep1 |
| GSM6341917 |
Metastatic mouse UPS cell lacking Setd1a, H3K4Me3, rep2 |
| GSM6341918 |
Metastatic mouse UPS cell, IgG, rep1 |
| GSM6341921 |
Metastatic mouse UPS cell, IgG, rep2 |
| GSM6341922 |
Metastatic mouse UPS cell, IgG, rep3 |
| GSM6341923 |
Metastatic mouse UPS cell lacking Setd1a, IgG, rep1 |
| GSM6341924 |
Metastatic mouse UPS cell lacking Setd1a, IgG, rep2 |
| GSM6341925 |
Metastatic mouse UPS cell lacking Setd1a, IgG, rep3 |
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| This SubSeries is part of SuperSeries: |
| GSE208367 |
COMPASS complex inhibition targets the metastasis initiating cell in undifferentiated pleomorphic sarcoma |
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| Relations |
| BioProject |
PRJNA859694 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE208365_RAW.tar |
851.7 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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