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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Aug 25, 2010 |
| Title |
PTIP promotes chromatin changes critical for immunoglobulin class switch recombination |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Programmed genetic rearrangements in lymphocytes require transcription at antigen receptor genes to promote accessibility for initiating double-strand break (DSB) formation critical for DNA recombination and repair. Here we show that activated B cells deficient in the PTIP component of the MLL3 (mixed-lineage leukemia 3) /MLL4 complex display impaired histone methylation (H3K4me3) and transcription initiation of downstream switch regions at the immunoglobulin heavy-chain (Igh) locus leading to defective immunoglobulin class-switching. We also show that PTIP accumulation at DSBs contributes to class-switch recombination (CSR) and genome stability independently from Igh switch transcription. These results demonstrate that PTIP promotes specific chromatin changes that control the accessibility of the Igh locus to CSR, and suggest a non-redundant role for the MLL3/MLL4 complex in altering antibody effector function.
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| Overall design |
Genome-wide analysis of histone modifications, PTIP, and Pol II in PTIP-WT and PTIP-KO mouse activated B cells.
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| Contributor(s) |
Daniel JA, Santos MA, Wang Z, Zang C, Schwab KR, Jankovic M, Filsuf D, Chen H, Gazumyan A, Yamane A, Cho Y, Sun H, Ge K, Peng W, Nussenzweig MC, Casellas R, Dressler GR, Zhao K, Nussenzweig A |
| Citation(s) |
20671152 |
| Submission date |
Mar 12, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Chongzhi Zang |
| Organization name |
University of Virginia
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| Street address |
P. O. Box 800717
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| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
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| Platforms (1) |
| GPL9185 |
Illumina Genome Analyzer (Mus musculus) |
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| Samples (31)
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| Relations |
| SRA |
SRP002152 |
| BioProject |
PRJNA124761 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20852_RAW.tar |
2.1 Gb |
(http)(custom) |
TAR (of BED, GRAPH) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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