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| Status |
Public on Mar 31, 2024 |
| Title |
Adenovirus small E1A directs activation of Alu transcription at YAP/TEAD- and AP-1- bound enhancers through interactions with the EP400 chromatin remodeler [ChIP-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Alu retrotransposons, forming the largest family of mobile DNA elements in the human genome, have recently come to attention as a potential source of regulatory novelties, most notably by participating in enhancer function. Even though Alu transcription by RNA polymerase III is subjected to tight epigenetic silencing, their bulk expression has long been known to increase in response to various types of stress, including viral infection. Here we show that, in primary human fibroblasts, adenovirus small e1a triggered derepression of hundreds of individual Alus, by promoting TFIIIB recruitment by Alu-bound TFIIIC. Epigenome profiling revealed an e1a-induced decrease of H3K27 acetylation and increase of H3K4 monomethylation at derepressed Alus, making them resemble poised enhancers. The enhancer nature of e1a-targeted Alus was confirmed by the enrichment, in their upstream regions, of the EP300/CBP acetyltransferase and of the YAP1 and FOS transcription factors. The physical interaction of e1a with the chromatin remodeler EP400 turned out to be critical for Alu derepression, and upstream enrichment of EP400 was found to commonly demarcate expression-prone Alus. Our data suggest that e1a targets a subset of enhancer Alus whose transcriptional activation, mediated by e1a-EP400 interaction, may participate in the manipulation of enhancer activity by adenovirus.
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| Overall design |
ChIP-seq of BDP1, Pol III and TFIIIC in G1-arrested IMR90 cells infected with HadV-C5 mutant dl1500 (which expresses the immediate early protein E1A with little or no expression of other viral genes). Mock-infected cells served as control.
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| Contributor(s) |
Cantarella S, Vezzoli M, Carnevali D, Morselli M, Zemke N, Montanini B, Daussy CF, Wodrich H, Teichmann M, Pellegrini M, Berk AJ, Dieci G, Ferrari R |
| Citation missing |
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| Submission date |
Jul 20, 2022 |
| Last update date |
Apr 27, 2024 |
| Contact name |
Giorgio Dieci |
| E-mail(s) |
giorgio.dieci@unipr.it
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| Phone |
+390521905649
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| Organization name |
University of Parma
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| Department |
Deparment of Life Sciences
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| Street address |
Parco Area delle Scienze 23/A
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| City |
Parma |
| ZIP/Postal code |
43124 |
| Country |
Italy |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE208717 |
Adenovirus small E1A directs activation of Alu transcription at YAP/TEAD- and AP-1- bound enhancers through interactions with the EP400 chromatin remodeler |
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| Relations |
| BioProject |
PRJNA860610 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE208685_RAW.tar |
2.2 Gb |
(http)(custom) |
TAR (of BED, BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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