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| Status |
Public on Mar 18, 2010 |
| Title |
Gene expression in NKR-P1B+ versus Ly49s3+ rat NK cells |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Two major subsets of rat natural killer (NK) cells can be distinguished based on their expression of either the Ly49s3 or the NKR-P1B lectin-like receptor. Ly49s3+ NK cells, but not NKR-P1B+ NK cells, express a wide range of Ly49 receptors.
Here, we performed a global gene expression profiling of sorted, single-positive NKR-P1B+ or Ly49s3+ splenic NK cells in order to get an overview of differences in expressed genes between the NKR-P1B+ and Ly49s3+ NK cell subsets.
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| Overall design |
NKR-P1B or Ly49s3 single-positive NK cells were sorted by flow cytometry from NK-enriched mononuclear cells from rat spleens. 9 rats of the PVG.7b strain aged 8-12 weeks were used. Total RNA was harvested by Tri Reagent extraction, and subjected to microarray analysis (Affymetrix Rat Genome 230 2.0 Array).
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| Contributor(s) |
Inngjerdingen M, Kveberg L |
| Citation(s) |
20395458 |
| Submission date |
Mar 17, 2010 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Marit Inngjerdingen |
| E-mail(s) |
marit.inngjerdingen@rr-research.no
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| Phone |
+4723073769
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| Fax |
+47230735 10
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| Organization name |
Oslo University Hospital, Rikshospitalet and University of Oslo
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| Department |
Institute of Immunology
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| Lab |
NK cell research group (prof. Vaage)
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| Street address |
Sognsvannsveien 20
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| City |
Oslo |
| ZIP/Postal code |
0027 |
| Country |
Norway |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA124379 |
