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| Status |
Public on Sep 13, 2023 |
| Title |
Characterization of a novel 3D-FN-silk breast cancer model |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To offer a robust and highly characterized three-dimensional (3D) model for anti-cancer drug discovery and basic research, we developed a 3D system in which the immortalized breast cancer cell lines MCF-7 and MDA-MB-231 were grown in recombinantly produced spider silk functionalized with the cell adhesion motif from fibronectin (FN-silk). The aim of this study is to evaluate whole-transcriptome changes driven by growth in such 3D model.
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| Overall design |
The expression profiling was generated using two immortalized breast cancer cell lines (MCF-7 and MDA-MB-231) cultured in two-dimensional (2D) or three-dimensional (3D) conditions for seven days. As a baseline control condition, cells obtained from canonical 2D culture on tissue-culture-treated flask on the day of the 3D scaffold creation (day 0) were used. For each condition, three replicates originating from independent experiments were considered as biological replicates. 18 samples were produced and analyzed.
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| Contributor(s) |
Caterina C, Hedhammar M, Hartman J, Chen X |
| Citation(s) |
37693069 |
| Submission date |
Jul 22, 2022 |
| Last update date |
Sep 13, 2023 |
| Contact name |
Caterina Collodet |
| Organization name |
KTH
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| Department |
Protein technology
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| Lab |
My Hedhammar
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| Street address |
Roslagstullsbacken 21
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| City |
Stockholm |
| State/province |
Stockholm |
| ZIP/Postal code |
11421 |
| Country |
Sweden |
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| Platforms (1) |
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| Samples (18)
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| GSM6376984 |
MCF7, 2D, Day 7, Biol rep 1 |
| GSM6376985 |
MCF7, 2D, Day 7, Biol rep 2 |
| GSM6376986 |
MCF7, 2D, Day 7, Biol rep 3 |
| GSM6376987 |
MCF7, 3D, Day 7, Biol rep 1 |
| GSM6376988 |
MCF7, 3D, Day 7, Biol rep 2 |
| GSM6376989 |
MCF7, 3D, Day 7, Biol rep 3 |
| GSM6376990 |
MDAMB231, Flask, Day 0, Biol rep 1 |
| GSM6376991 |
MDAMB231, Flask, Day 0, Biol rep 2 |
| GSM6376992 |
MDAMB231, Flask, Day 0, Biol rep 3 |
| GSM6376993 |
MDAMB231, 2D, Day 7, Biol rep 1 |
| GSM6376994 |
MDAMB231, 2D, Day 7, Biol rep 2 |
| GSM6376995 |
MDAMB231, 2D, Day 7, Biol rep 3 |
| GSM6376996 |
MDAMB231, 3D, Day 7, Biol rep 1 |
| GSM6376997 |
MDAMB231, 3D, Day 7, Biol rep 2 |
| GSM6376998 |
MDAMB231, 3D, Day 7, Biol rep 3 |
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| Relations |
| BioProject |
PRJNA861264 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE209570_MCF7matrix.txt.gz |
1009.0 Kb |
(ftp)(http) |
TXT |
| GSE209570_MDAMB231matrix.txt.gz |
1.4 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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