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| Status |
Public on Oct 31, 2022 |
| Title |
Comprehensive analysis of M2 macrophage-derived exosomes facilitating osteogenic differentiation of human periodontal ligament stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
As the primary seed cells in periodontal tissue engineering, the role of periodontal ligament stem cells (PDLSCs) in periodontal tissue regeneration and bone remodeling during orthodontic tooth movement (OTM) has been well documented. Nevertheless, the impact of different polarization states of macrophages on the osteogenic differentiation of PDLSCs is poorly understood. M0, M1 and M2 macrophage-derived exosomes (M0-exo, M1-exo and M2-exo) were treated with primary cultured human PDLSCs, respectively. Identification of differentially expressed microRNAs (DE-miRNA) in M0-exo and M2-exo by miRNA microarray. In summary, we have indicated for the first time that M2-exo can promote osteogenic differentiation of human PDLSCs, and have revealed the functions and pathways involved in the DE-miRNAs of M0-exo and M2-exo and their downstream targets.
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| Overall design |
M0, M1 and M2 macrophage-derived exosomes (M0-exo, M1-exo and M2-exo) were treated with primary cultured human PDLSCs, respectively. Identification of differentially expressed microRNAs (DE-miRNA) in M0-exo and M2-exo by miRNA microarray.
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| Contributor(s) |
Hu J, Liao X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 28, 2022 |
| Last update date |
Nov 02, 2022 |
| Contact name |
Jiang-Tian Hu |
| E-mail(s) |
Prof_jiangtian_hu@outlook.com
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| Organization name |
Yunnan Stomatology Hospital
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| Street address |
No. 1088 Middle Haiyuan Road
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| City |
Kunming |
| State/province |
Yunnan |
| ZIP/Postal code |
650106 |
| Country |
China |
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| Platforms (1) |
| GPL25134 |
Agilent-070156 Human_miRNA_V21.0_Microarray 046064 (miRNA ID version) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA863226 |
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