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| Status |
Public on Sep 01, 2023 |
| Title |
Osteoclast microRNA profiling to capture the erosive factor in rheumatoid arthritis |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
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| Summary |
Background: In rheumatoid arthritis (RA), only a subset of patients develop an irreversible bone destruction. Our aim was to develop a microRNA (miR)-based osteoclast-related signature to predict erosiveness in RA.
Results: We identified 5 miRs from PBMC-derived osteoclasts differentially expressed in RNASeq also in qPCR assessment.
Conclusion: We found 5 miRNAs differentially refulated in erosive rheumtoid arhtritis compare to no erosve patient. This study could improve the current approach to identify RA subjects at risk of erosions, by adding biomarker signatures based on the profile of miRs in the osteoclasts.
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| Overall design |
Blood samples were obtained from 8 patients with erosive and 8 with no erosive RA sex- and age- matched controls. Peripheral blood mononuclear cells (PBMCs) were differentiated into osteoclasts in 3-week in-vitro cultures and osteoclast samples were sent to be sequenced using multiplex sequencing on a HiSeq (Illumina) instrument (Genome Quebec).
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| Contributor(s) |
Nguyen HD, Lortie A, Mbous Nguimbus L, Marrugo J, Allard-Chamard H, Bouchard L, Boire G, Scott MS, Roux S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 28, 2022 |
| Last update date |
Sep 01, 2023 |
| Contact name |
Sophie Roux |
| E-mail(s) |
Sophie.Roux@USherbrooke.ca
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| Organization name |
Université de Sherbrooke
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| Department |
Division of Rheumatology
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| Street address |
3001, 12th avenue N
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| City |
Sherbrooke |
| State/province |
Quebec |
| ZIP/Postal code |
J1H5N4 |
| Country |
Canada |
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| Platforms (1) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA863270 |
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