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| Status |
Public on Aug 15, 2022 |
| Title |
Integrated multi-omics approach revealed cellular senescence landscape |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Cellular senescence is a complex multifactorial biological phenomenon that plays essential roles in aging, and aging-related diseases. During this process, the senescent cells undergo gene expression altering and chromatin structure remodeling. However, studies on the epigenetic landscape of senescence using integrated multi-omics approaches are limited. In this research, we performed ATAC-seq, RNA-seq, and ChIP-seq on different senescent types to reveal the landscape of senescence and identify the prime regulatory elements.
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| Overall design |
Considering that human diploid 2BS fibroblasts are a widely used cell model to study cellular senescence and aging-related disorders, we employed growing 2BS cells and senescent 2BS cells as Replicative Cellular Senescence (RS) representatives to generate ATAC-seq libraries , which can sensitively measure high-resolution chromatin accessibility. For each age stage, we obtained two independent ATAC-seq replicates.
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| Web link |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9638896/
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| Contributor(s) |
Wang P, Song Q, Wang C, Zhang L, Wei G, Hou Y, Liu J, Wang Y, Cui Y |
| Citation(s) |
36243980 |
| Submission date |
Aug 02, 2022 |
| Last update date |
Nov 21, 2022 |
| Contact name |
Qiao Song |
| E-mail(s) |
songqiao@ccmu.edu.cn
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| Phone |
13521570356
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| Organization name |
Xuanwu Hospital, Capital Medical University
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| Department |
Department of Clinical laboratory
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| Lab |
National Clinical Research Center for Geriatric Diseases
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| Street address |
No.45 Changchun Street
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| City |
Beijing |
| ZIP/Postal code |
100053 |
| Country |
China |
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| Platforms (2) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA865031 |
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