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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 03, 2010 |
| Title |
Express data from Du145 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The retinoblastoma (Rb) tumor suppressor is often inactivated in cancers. To identify genes that can be used to specifically target such cancers, we carried out a genetic screen in Drosophila. We identified gig (fly TSC2) and found that inactivation of rbf (fly Rb) and gig synergistically induced cell death. Interestingly, inactivation of TSC2 specifically kills Rb mutant cancer cells under stress conditions, which is correlated with an inhibition of tumor growth. We show that cancer cell killing induced by concomitant inactivation of Rb and TSC2 is mediated by increased cellular stress, including oxidative stress. Inactivation of TSC2 and Rb synergistically induce oxidative stress via increased protein synthesis, inhibited de novo lipid synthesis, and decreased ROS scavenger enzyme SOD2 induction.
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| Overall design |
Du145 was treated by shTSC2 or control, and then cultured in hypoxic conditions for 24h, and finally the total RNA was collected for microarray analysis.
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| Contributor(s) |
Du W, Li B |
| Citation(s) |
20478529 |
| Submission date |
Mar 31, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Wei Du |
| E-mail(s) |
wei@uchicago.edu
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| Phone |
773-834-2141
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| Organization name |
University of Chicago
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| Street address |
929 E. 57th Street
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| City |
chicago. |
| ZIP/Postal code |
60637 |
| Country |
USA |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA126671 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21147_RAW.tar |
26.3 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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