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| Status |
Public on Nov 16, 2022 |
| Title |
Transcriptome analysis between wild-type and DM1-affected fibroblast. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Human skin fibroblast cell lines from 3 normal individuals and 3 individuals with DM1 in one pedigree were obtained from the biobank of Shanghai East Hospital. Human skin fibroblast cells were grown in DMEM (Life Technologies), NaPyr, GlutaMAX, β-mercaptoethanol and 10% foetal bovine serum (FBS) supplemented medium as previously described (Richner et al. 2015). Cells were collected after 80% confluency with TRIzol reagent and stored at -80℃ for RNA-seq and RT-PCR.
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| |
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| Overall design |
Comparative gene expression profiling analysis of RNA-seq data for wild-type and DM1-affected fibroblast.
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| Contributor(s) |
Hu M, Zhang B, Li G |
| Citation(s) |
36338967 |
| Submission date |
Aug 17, 2022 |
| Last update date |
Nov 16, 2022 |
| Contact name |
Min Hu |
| E-mail(s) |
hm2021cns@gmail.com
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| Organization name |
Tongji University School of Medicine
|
| Street address |
1800 Yuntai Road, Shanghai 200123, China
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| City |
Shanghai |
| State/province |
Select a State or Province |
| ZIP/Postal code |
200123 |
| Country |
China |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA870504 |
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