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| Status |
Public on Apr 13, 2010 |
| Title |
Characterization of the RNA content of chromatin |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harbouring CARS.
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| Overall design |
We purified CARs from normal HFs by isolating soluble chromatin after MNase treatment, followed by separation of chromatin fragments of different lengths on a sucrose gradient. CARs were converted into double-stranded cDNAs and sequenced using the Illumina Genome Analyzer I.
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| Contributor(s) |
Mondal T, Rasmussen M, Pandey GK, Isaksson A, Kanduri C |
| Citation(s) |
20404130 |
| Submission date |
Apr 06, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Anders Isaksson |
| E-mail(s) |
anders.isaksson@medsci.uu.se
|
| Organization name |
Uppsala University
|
| Street address |
Akademiska sjukhuset ing61 3tr
|
| City |
Uppsala |
| ZIP/Postal code |
75185 |
| Country |
Sweden |
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| Platforms (1) |
| GPL9052 |
Illumina Genome Analyzer (Homo sapiens) |
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| Samples (1) |
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| Relations |
| SRA |
SRP002245 |
| BioProject |
PRJNA126491 |
