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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Jun 21, 2010 |
| Title |
Expression data from C2C12 mouse myoblast with treatment actinomycin D |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dramatic changes in gene expression occur in response to extracellular stimuli and during differentiation. Although transcriptional effects are important, alterations in mRNA decay also play a major role in achieving rapid and massive changes in mRNA abundance. Moreover, just as transcription factor activity varies between different cell types, the factors influencing mRNA decay are also cell-type specific. We have established the rates of decay for over 7000 transcripts expressed in mouse C2C12 myoblasts.
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| Overall design |
To estimate mRNA decay rate in mouse muscle cells, we treated C2C12 mouse myoblasts with actinomycin D to inhibit transcription and collected samples at 0, 10, 50, 110 and 230 min.
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| Contributor(s) |
Lee JE, Lee JY, Wilusz J, Tian B, Wilusz CJ |
| Citation(s) |
20574513 |
| Submission date |
Apr 07, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Ju Youn Lee |
| Organization name |
UMDNJ-New Jersey Medical School
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| Department |
Biochemistry
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| Street address |
185 South Orange Ave. MSB-E501
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| City |
Newark |
| State/province |
NJ |
| ZIP/Postal code |
07101 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (15)
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| GSM530900 |
C2C12 with actinomycin D, time 0 min, rep 1 |
| GSM530901 |
C2C12 with actinomycin D, time 0 min, rep 2 |
| GSM530902 |
C2C12 with actinomycin D, time 0 min, rep 3 |
| GSM530903 |
C2C12 with actinomycin D, time 10 min, rep 1 |
| GSM530904 |
C2C12 with actinomycin D, time 10 min, rep 2 |
| GSM530905 |
C2C12 with actinomycin D, time 10 min, rep 3 |
| GSM530906 |
C2C12 with actinomycin D, time 50 min, rep 1 |
| GSM530907 |
C2C12 with actinomycin D, time 50 min, rep 2 |
| GSM530908 |
C2C12 with actinomycin D, time 50 min, rep 3 |
| GSM530909 |
C2C12 with actinomycin D, time 110 min, rep 1 |
| GSM530910 |
C2C12 with actinomycin D, time 110 min, rep 2 |
| GSM530911 |
C2C12 with actinomycin D, time 110 min, rep 3 |
| GSM530912 |
C2C12 with actinomycin D, time 230 min, rep 1 |
| GSM530913 |
C2C12 with actinomycin D, time 230 min, rep 2 |
| GSM530914 |
C2C12 with actinomycin D, time 230 min, rep 3 |
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| This SubSeries is part of SuperSeries: |
| GSE21236 |
Systematic analysis of cis-elements in unstable mRNAs demonstrates that CUGBP1 is a key regulator of mRNA decay in muscle cells |
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| Relations |
| BioProject |
PRJNA129711 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21233_RAW.tar |
98.0 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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