 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2011 |
| Title |
Genome-wide analysis of matched microRNA-mRNA time-course data after androgen injection to prostate cancer LNCaP cell line |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Non-coding RNA profiling by array
|
| Summary |
Androgen receptor (AR) signalling pathway plays an important role in carcinogenesis and development of prostate cancer. The involvement of microRNA (miRNA) in this process is still largely unknown. In this study, we performed a matched miRNA-mRNA time-course expression profiling to reveal androgen response in hormone-sensitive prostate cancer cells.We introduced novel statistics Response Score (RS) and Modulation Score (MS) to identify significant androgen-regulated target genes and miRNA-modulated target mRNAs. Based on the analysis, we found several novel androgen-regulated targets, which had significant androgen response in expression pattern, and were highly enriched in predicted androgen responsive elements (AREs). AR-bindings to these AREs were validated with ChIP assay. Furthermore, a set of target mRNAs involved in crucial processes of tumor progression were identified to be significantly regulated by these miRNAs. Therefore, a miRNA-mediated androgen signalling network was inferred, including three novel feedback mechanisms for AR self-modulation. In conclusion, our study provides new approaches to further miRNA regulation research and contributes novel findings into miRNA-mediated pathological effects in prostate cancer.
|
| |
|
| Overall design |
Total RNA obtained from androgen dihydrotestosterone (DHT) subjected to LNCaP cells in vitro at 20min, 40min, 1h, 2h, 4h, 8h, 16h, 24h and 48h, compared to the control at 0h.
|
| |
|
| Contributor(s) |
Mo W, Zhang J |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 07, 2010 |
| Last update date |
Feb 18, 2019 |
| Contact name |
Wenjuan Mo |
| E-mail(s) |
041019012@fudan.edu.cn
|
| Phone |
021-65100243
|
| Fax |
021-65100243
|
| Organization name |
Fudan University
|
| Department |
Life School
|
| Lab |
Genetics national lab
|
| Street address |
No. 220 Handan Road
|
| City |
Shanghai |
| ZIP/Postal code |
200433 |
| Country |
China |
| |
|
| Platforms (2) |
| GPL6884 |
Illumina HumanWG-6 v3.0 expression beadchip |
| GPL8933 |
Illumina Human Beta-version microRNA expression BeadChip, |
|
| Samples (20)
|
| GSM531026 |
total microRNAs in LNCaP cells treated with DHT at the beginning |
| GSM531027 |
total microRNAs in LNCaP cells treated with DHT at 20min |
| GSM531028 |
total microRNAs in LNCaP cells treated with DHT at 40min |
| GSM531029 |
total microRNAs in LNCaP cells treated with DHT at 1h |
| GSM531030 |
total microRNAs in LNCaP cells treated with DHT at 2h |
| GSM531031 |
total microRNAs in LNCaP cells treated with DHT at 4h |
| GSM531032 |
total microRNAs in LNCaP cells treated with DHT at 8h |
| GSM531033 |
total microRNAs in LNCaP cells treated with DHT at 16h |
| GSM531034 |
total microRNAs in LNCaP cells treated with DHT at 24h |
| GSM531035 |
total microRNAs in LNCaP cells treated with DHT at 48h |
| GSM531036 |
total mRNAs in LNCaP cells treated with DHT at the beginning |
| GSM531037 |
total mRNAs in LNCaP cells treated with DHT at 20min |
| GSM531038 |
total mRNAs in LNCaP cells treated with DHT at 40min |
| GSM531039 |
total mRNAs in LNCaP cells treated with DHT at 1h |
| GSM531040 |
total mRNAs in LNCaP cells treated with DHT at 2h |
| GSM531041 |
total mRNAs in LNCaP cells treated with DHT at 4h |
| GSM531042 |
total mRNAs in LNCaP cells treated with DHT at 8h |
| GSM531043 |
total mRNAs in LNCaP cells treated with DHT at 16h |
| GSM531044 |
total mRNAs in LNCaP cells treated with DHT at 24h |
| GSM531045 |
total mRNAs in LNCaP cells treated with DHT at 48h |
|
| Relations |
| BioProject |
PRJNA126519 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE21245_RAW.tar |
6.3 Mb |
(http)(custom) |
TAR |
| GSE21245_mRNA_non-normalized.txt.gz |
3.5 Mb |
(ftp)(http) |
TXT |
| GSE21245_miRNA_non-normalized.txt.gz |
62.8 Kb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...